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Written as an advanced text for toxicology students, this book is much more than an introduction and provides in-depth information describing the underlying mechanisms through which toxicants produce their adverse responses. * Links traditional toxicology to modern molecular techniques, important for teaching to graduate courses and professional studies * Uses a didactic approach with basic biological or theoretical background for the methodology presented * Brings together and comprehensively covers a range of dynamic aspects in biochemical and molecular toxicology * Guides student and…mehr
Written as an advanced text for toxicology students, this book is much more than an introduction and provides in-depth information describing the underlying mechanisms through which toxicants produce their adverse responses. * Links traditional toxicology to modern molecular techniques, important for teaching to graduate courses and professional studies * Uses a didactic approach with basic biological or theoretical background for the methodology presented * Brings together and comprehensively covers a range of dynamic aspects in biochemical and molecular toxicology * Guides student and professional toxicologists in comprehending a broad range of issues, compiled and authored by a diverse group of experts * "A good introductory textbook covering the biochemical toxicology of organic substances and the relevant methodology in some detail.... It offers good value for money and can be recommended as a textbook for appropriate courses" - BTS Newsletter review of the 4th edition
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Autorenporträt
ROBERT C. SMART, PhD, is a William Neal Reynolds Professor of Toxicology, the Director of the Center for Human Health and the Environment, and the former Director of the Toxicology Graduate Program at North Carolina State University. Together with Dr. Hodgson, he coedited the two previous editions of this book. ERNEST HODGSON, PhD, is Distinguished Professor Emeritus of Environmental and Molecular Toxicology and Executive Director of the Foundation for Agromedicine and Toxicology at North Carolina State University. Alongside the previous editions of this book, he edited four editions of A Textbook of Modern Toxicology (Wiley) and is the Editor of the Journal of Biochemical and Molecular Toxicology.
Inhaltsangabe
Preface xxix
List of Contributors xxxi
Section 1 Introduction 1
1 Molecular and Biochemical Toxicology: Definition and Scope 3 Ernest Hodgson and Robert C. Smart
1.1 Introduction 3
1.2 Sources of Information 5
1.3 Toxicology 5
1.4 Molecular and Cellular Toxicology 6
1.5 Proteomics and Metabolomics 8
1.6 Role of Molecular, Cellular, and Biochemical Toxicology: Implications for Risk Assessment 8
1.7 Conclusions 9
Suggested Reading 9
Section 2 Techniques in Biochemical and Molecular Toxicology 11
2 Molecular Techniques for the Study of Gene Function 13 Yoshiaki Tsuji and Robert C. Smart
2.1 Applicability of Molecular Techniques to Toxicology 13
2.2 Overview of Genes, Chromatin, and Their Relationship 15
2.3 Approaches to Characterize the Functions of Genes 20
2.4 Gene Targeting Technologies 31
2.5 Analysis of Gene Expression and Regulatory Mechanisms 35
2.6 Methods to Evaluate Gene Function in an Animal Model 45
References 54
Suggested Reading 54
3 Transcriptomics 55 B. Alex Merrick
3.1 Introduction 55
3.2 Cellular Organization and the Transcriptome 58
3.3 RNA Isolation 62
3.4 Platforms for Transcriptome Analysis: Microarrays 64
3.5 RNA?]seq: NextGen Sequencing of the Transcriptome 72
3.6 Validation of Transcriptome Analysis 81
3.7 Analysis of Gene Expression Data 82
3.8 Summary 86
References 87
4 Proteomics 91 Michael S. Bereman
4.1 Introduction to Proteomics 91
4.2 Mass Spectrometry 94
4.3 Quantitation of Proteins by LC?]MS/MS 107
4.4 Emerging Research Areas in Proteomics 109
4.5 Summary 112
Suggested
Reading 112
5 Metabolomics 115 Susan C. J. Sumner, Wimal Pathmasiri, James E. Carlson, Susan L. McRitchie, and Timothy R. Fennell
5.1 Introduction 115
5.2 Endogenous and Exogenous Metabolites 116
5.3 Study Design and Experimental Considerations 117
5.4 Applications of Metabolomics 120
5.5 Technologies for Targeted and Broad?]Spectrum Metabolomics 122
5.6 Statistical and Multivariate Analysis and Pathway Mapping 127
5.7 Summary 130
Acknowledgment 130
References 131
6 Cellular Techniques 135 Sharon A. Meyer and Barbara A. Wetmore
6.1 Introduction 135
6.2 Cellular Studies in Intact Tissue 137
6.3 Studies
6.4 Monolayer Cell Culture 142
6.5 Observation of Cultured Cells 148
6.6 Indicators of Toxicity 149
6.7 Important Considerations and Advances 152
6.8 Replacement of Animal Testing with Cell Culture Models 155
6.9 Conclusion 157
Suggested Reading 157
Journals 158
7 Basic Concepts of Molecular Epidemiological Research Methods 159 Cathrine Hoyo, David A. Skaar, and Randy L. Jirtle
7.1 Introduction 159
7.2 Molecular Epidemiology 160
7.3 Descriptive Epidemiologic Study Designs 164
7.4 Analytic Epidemiologic Studies 167
7.5 Experimental Studies 173
7.6 Inferring Causality from Molecular Epidemiologic Data 174
7.7 Summary 182
References 183
Suggested Reading 184
Reading Materials Related to Examples 184
Section 3 Mechanisms in Molecular and Biochemical Toxicology 187
8 Phase I and Phase II Metabolism and Metabolic Interactions: A Summary 189 Ernest Hodgson
