Molecular Diagnostics (eBook, ePUB)

Redaktion: Patrinos, George P.; Ansorge, Wilhelm

• Format: ePub

Produktbeschreibung

Molecular Diagnostics covers current molecular biological techniques used to identify the underlying molecular defects in inherited disease. Although an increasing number of laboratories, both academic and private are moving in that direction, there are only a few books in the existing literature, and they deal only partly with diagnosis at the molecular level. Each chapter includes the principle and a brief description of the technique, followed by exmples from the authors' own epertise. Contributors are well-known experts in their field, and derive from a variety of disciplines, to ensure breadth and depth of coverage.

• Examines widely used molecular biology techniques to screen for genetic defects causing inherited disorders
• Includes state-of-the-art techniques for the detection of the underlying genetic heterogeneity leading to inherited disorders
• Identification of genetically modified organisms (GMO's)
• Forensic analysis and every-day issues in a diagnostic laboratory
• Discusses ethics, genetic counselling and quality management

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 488
• Erscheinungstermin: 6. Juni 2005
• Englisch
• ISBN-13: 9780080473505
• Artikelnr.: 38116193

Autorenporträt

Dr. George Patrinos is a Senior Research Scientist in Erasmus University Medical Center in Rotterdam, the Netherlands. He has many years of research experience in the field of Molecular Diagnostics. One of his current research interests is high throughput mutation screening and development of National mutation databases as online diagnostic tools.
Prof. Dr. Wilhelm Ansorge is a Senior Research Scientist and coordinator of the Biochemical Instrumentation Programme at the European Molecular Biology Laboratory in Heidelberg, Germany. His research interests include the development of the first complete Human Genome microarray, with numerous applications in gene expression studies and high-throughput Molecular Diagnostics.

Inhaltsangabe

Chapter 1: Molecular Diagnostics: Past, Present, and Future
Section I: Molecular Diagnostic Technology
Chapter 2: Allele-Specific Mutation Detection by PCR-ARMS and PCR-ASO
Chapter 3: Competitive Oligopriming
Chapter 4: Oligonucleotide Ligation Assays for the Diagnosis of Inherited
Diseases
Chapter 5: Enzymatic and Chemical Cleavage Methods to Identify Genetic
Variation
Chapter 6: Mutation Detection by Single Strand Conformation Polymorphism
and Heteroduplex Analysis
Chapter 7: Capillary Electrophoresis
Chapter 8: Temperature and Denaturing Gradient Gel Electrophoresis
Chapter 9: Two-Dimensional Gene Scanning
Chapter 10: Real-Time Polymerase Chain Reaction
Chapter 11: Pyrosequencing
Chapter 12: Molecular Cytogenetics in Molecular Diagnostics
Chapter 13: Detection of Genomic Duplications and Deletions
Chapter 14: Analysis of Human Splicing Defects Using Hybrid Minigenes
Chapter 15: DNA Microarrays and Genetic Testing
Chapter 16: Human Genome Microarray in Biomedical Applications
Chapter 17: Use of High Throughput Mass Spectrographic Methods to Identify
Disease Processes
Chapter 18: The Application of Proteomics to Disease Diagnostics
Section II: Applications of Molecular Diagnostics and Related Issues
Chapter 19: Identification of Genetically Modified Organisms
Chapter 20: Pharmacogenetics and Pharmacogenomics: Impact on Drug Discovery
and Development
Chapter 21: Molecular Diagnostic Applications in Forensic Science
Chapter 22: Molecular Diagnostics and Comparative Genomics in Clinical
Microbiology
Chapter 23: Preimplantation Genetic Diagnosis
Chapter 24: Genetic Monitoring of Laboratory Animals
Chapter 25: The Use of Locus-Specific Databases in Molecular Diagnostics
Chapter 26: Safety Analysis in Retroviral Gene Therapy: Identifying Virus
Integration Sites in Gene-Modified Cells
Chapter 27: Automated DNA Hybridization and Detection
Chapter 28: The Use of Microelectronic-Based Techniques in Molecular
Diagnostic Assays
Chapter 29: Miniaturization Technologies for Molecular Diagnostics
Chapter 30: Human Gene Patents and Genetic Testing
Chapter 31: Genetic Counselling and Ethics in Molecular Diagnostics
Chapter 32: Genetic Testing and Psychology
Chapter 33: Safety in Biomedical and Other Laboratories
Chapter 34: Quality Management in the Laboratory