Neuropeptide Y Protocols (eBook, PDF)

Redaktion: Balasubramaniam, Ambikaipakan

• Format: PDF

Produktbeschreibung

It is now clear that neuropeptide Y (NPY), the most abundant peptide present in the mammalian nervous system, is implicated in the pathophysiology of many diseases, including eating disorders, seizures, memory loss, anxiety, depression, and cardiovascular diseases, and offers major therapeutic opportunities for their treatment. In Neuropeptide Y Protocols, a strong cohort of established investigators from a variety of disciplines detail their best techniques for advancing NPY research. Among the many readily reproducible methods described are those for cloning, for the production of transgenic and knockout models, and for the use of antisense technology to define the receptors and the signal transduction pathways mediating NPY actions. A range of the major qualitative and quantitative techniques used to study tissue mRNA distribution are also included, as well as methods for radioligand binding. The cloning techniques utilize genomic DNA isolation, expression cloning, PCR cloning, the construction of hybrid receptors, and homology-based cloning. Highly practical and state-of-the-art, Neuropeptide Y Protocols constitutes a gold-standard collection of proven techniques for advancing today's understanding of this significant neuropeptide, as well as for developing the promising NPY-based therapeutics now emerging for many important disorders.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 246
• Erscheinungstermin: 5. Februar 2008
• Englisch
• ISBN-13: 9781592590421
• Artikelnr.: 64701124

Inhaltsangabe

Cloning Techniques.- Cloning Neuropeptide Tyrosine cDNA.- Human Y1/Y5 Receptor Gene Cluster.- Human Type 2 Neuropeptide Y Receptor Gene.- Y4 Receptor in Different Species.- NeuropeptideYY5 Receptor Expression.- Homology-Based Cloning Methods.- Production of Transgenic and Knock-Out Models.- Developing Transgenic NeuropeptideY Rats.- NeuropeptideYY1 Receptor-Deficient Mice.- Antisense Technology.- Antisense Oligonucleotide Approach to Study NPY-Mediated Feeding Signal Transduction.- A G?i RNA-Antisense Expression Strategy to Investigate Coupling of Peptide YY/Neuropeptide Y Receptor to G?i.- NPY Y5 Receptor Subtype.- NPY Antisense Oligodeoxynucleotides to Study the Actions of NPY.- Techniques to Study Tissue Distribution of mRNA.- Localization of Y-Receptor Subtype mRNAs in Rat Brain by Digoxigenin Labeled In Situ Hybridization.- Central Y4 Receptor Distribution.- Combining Non-Isotopic Localization of NPY mRNA with Immunocytochemistry.- Miscellaneous Techniques.- Y Receptors Characterized by RT-PCR.- Quantification of NPY mRNA by Ribonuclease Protection Assay.- Radioligand Binding Studies.

Rezensionen

"...the series is necessary in any library that supports a molecular biology/genetics program...Methods in Molecular Biology is indexed in PubMed, making it very easy to find the protocols. This volume covers neuropeptide Y (NYP), the most abundant peptide in the mammalian nervous system. NYP has also been implicated in the pathophysiology of diseases such as seizures, memory loss, anxiety, and depression, making it an important molecule in clinical research as well. As such, this volume is excellent for any neurobiology or medical collection. The editor of the volume, Ambikaipakan Balasubramaniam, is a well-published researcher in the field"-E-Streams "The editor of this monograph is to be congratulated on bringing together a group of leading experts in the NPY field to describe in a 'user friendly', practical way how to investigate the properties of this peptide....this is an excellent 'cookbook' that will appeal to both the basic and clinical neuroscientist who wishes to study the physiological or pathological effects of this important neuropeptide. The book is nicely produced and the cloth binding gives it the added robustness necessary should it be used at the laboratory bench!"-Human Phychopharmacology "The protocols in this volume cover a wide variety of techniques, from cloning and isolation, to expression and localization. Despite this wide range, each chapter give a quick introduction to the technique and thoroughly discusses the procedures. Methods and materials are specific and clear. A favorite part of all the protocols is the "Notes" section that gives tips and ideas for avoiding some of the usual pitfalls of the technique. Where applicable there are also shortcuts or other ideas to make the protocol work smoothly. The reference section is useful, oftentimes giving early, "historic" references for a technique. There is an extensive index and numerous illustrations, charts and tables to clarify the techniques and results." - E-Streams "This is the first handbook on NPY research techniques and the protocols described in this comprehensive collection can be easily extrapolated to study any peptide hormone, therefore making this volume beneficial to all investigators involved in polypeptide hormone research." - Lavoisier Librairie