Neurotrophin Protocols (eBook, PDF)

Redaktion: Rush, Robert A.

• Format: PDF

Produktbeschreibung

Despite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular localization and quantification of the neurotrophins, to genetic manipulation of cells and animals for the analysis of biological function, to quantitative analysis of the active neurotrophin genes. There are also radiotracing techniques for studying neurotrophin transport in both the retrograde and anterograde directions, procedures for using immunotoxins to study the effects of eliminating a single class of neurons, and the essential stereological method for estimation of neuronal numbers. Each method includes not only detailed step-by-step instructions, but also a list of necessary equipment and supplies, and valuable notes spelling out quick tips and tricks of the trade. Timely and robust, Neurotrophin Protocols provides today's neuroscientists in both academia and industry with a comprehensive range of practical, readily reproducible methods for studying neurotrophins and the critically important effects they have on the nervous system.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 274
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781592590605
• Artikelnr.: 64692024

Inhaltsangabe

Part 1. Neurotrophin Quantification and Localization. Localization of Neurotrophin Proteins Within the Central Nervous System by Immmunohistochemistry, James M. Conner. Neurotrophin Immunohistochemistry in Peripheral Tissues, Robert A. Rush and Xin-Fu Zhou. Extraction and Quantification of the Neurotrophins, Shu-Hua Zhang, Xin-Fu Zhou, and Robert A. Rush. Identifying Novel Proteins in Nervous Tissue Using Microsequencing Techniques, Selena E. Bartlett. Part 2. RNA Quantification and Localization. Quantification of mRNA Levels Using Northern Blotting, Paul Fernyhough. Quantification of mRNA Levels Using Ribonuclease Protection Assay, Pirkko Henttu. Quantification of Neurotrophin mRNA by RT-PCR, Edward Chie, Dennis Liu, Xin-Fu Zhou, and Robert A. Rush. Sensitive and Nonradioactive In Situ Detection of Neurotrophin mRNAs in the Nervous System, Cory J. Xian and Xin-Fu Zhou. Part 3. Construction of Recombinant Proteins, and Transgenic Cells and Animals. The Expression of Neurotrophic Factors such as GDNF in the E. coli System, Bing-Ren Huang and Xue-Mei Ma. Construction of Cells Expressing Neurotrophins, Jasodhara Ray and Fred H. Gage. Efficient Generation of Stable Pheochromocytoma (PC12) Cell Lines Using a Recombinant Retrovirus (LNC), Matthew E. Cunningham, Jan K. Kitajewski, and Lloyd A. Greene. Construction and Analysis of Transgenic Animals Overexpressing Neurotrophins, Kathryn M. Albers and Brian M. Davis. Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors, Carrie G. Causing and Freda D. Miller. Part 4. In Vivo Analysis. Tracing with Radiolabeled Neurotrophins, Christopher S. von Bartheld. Toxin-Induced Death of Neurotrophin-Sensitive Neurons, Ronald G. Wiley. Oligonucleotides as Inhibitors of Protein Synthesis, W. Ruwan Epa, Graham L. Barrett, and Perry F. Bartlett. The IODO-GEN Method for Labeling and the Use of Retrograde Axonal Transport to Assay Neurotrophins, Ian A. Hendry and Anna J. Reynolds. Stereological Estimation of Neur.

Rezensionen

"...provides a collection of chapters written by experts in developmental neuroscience, neuroanatomy, signaling mechanisms that regulate neuronal survival and plasticity, and neurodegenerative disorders....This book will be a valuable addition to libraries at Universities and various other institutes engaged in biomedical sciences, as well as to the personal collection of those scientists interested in growth factors....A strength of Neurotrophin Protocols is that it covers considerable ground with little repetition of background information and specific methods among chapters....we expect that this book will spend less time on the shelf and more time on lab benches and office desks. This is well done and timely contribution to the field of neurotrophic factor research."-Journal of Molecular Neuroscience