p53 Protocols (eBook, PDF)

Redaktion: Deb, Sumitra; Deb, Swati Palit

• Format: PDF

Produktbeschreibung

Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal its unique structural features and biochemical functions. In p53 Protocols, Sumitra and Swati Palit Deb have assembled an indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. Described by leading basic and clinical researchers who have successfully used the methods, the techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation. Special effort has been made to discuss problems and their solutions to help ease the learning curve when standardizing a new method. Diverse and highly practical, p53 Protocols offers both beginning and experienced researchers in cancer biology a gold-standard compendium of readily reproducible techniques for studying p53 and related proteins.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 280
• Erscheinungstermin: 2. Februar 2008
• Englisch
• ISBN-13: 9781592594085
• Artikelnr.: 64559585

Inhaltsangabe

Adenovirus Expressing p53.- Purification of Recombinant p53 from Sf9 Insect Cells.- Generation and Characterization of p53 Mutant Mice.- Identification of p53 Target Genes by Fluorescent Differential Display.- p53-Induced Gene Expression Analysis.- Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Using SYBR® Green I.- Transactivation and Transrepression Studies with p53.- Methods to Study p53-Repressed Promoters.- Interaction of p53 with Cellular Proteins.- Interaction of p53 with the Adenovirus E1B-55 kDa Protein.- In Vivo Footprinting and DNA Affinity Chromatography for Analysis of p53 DNA Binding Ability.- Signaling to p53.- Detection of Mitochondrial Localization of p53.- Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress.- Mutational Analysis of p53 in Human Tumors.- Mutational Analysis of p53 in Human Tumors.- Flow Cytometric Analysis of p53-Induced Apoptosis.- Flow Cytometric Analysis of MDM2-Mediated Growth Arrest.