PCR Revolution (eBook, PDF)

Basic Technologies and Applications
Redaktion: Bustin, Stephen A.

• Format: PDF

Produktbeschreibung

The invention of the polymerase chain reaction (PCR) won the Nobel Prize in Chemistry in 1994 and remains one of the most important scientific discoveries of the twentieth century. More than 50,000 researchers in the United States use PCR replication technology, yet there is a significant absence of any recent publications on this subject. In this book, Dr Stephen A. Bustin, a world-renowned PCR expert, examines in detail the latest innovations and the overall impact of PCR on many areas of molecular research. The book contains personal reflections, opinions, and comments by leading authorities on the many applications of the PCR and how this technology has revolutionised their respective areas of interest. Bustin explains the ways in which PCR has overcome many obstacles in life science and clinical research and charts the PCR's development from time-consuming, low throughput, non-quantitative procedure to today's rapid, high throughput, quantitative super method.

---

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Produktdetails

• Verlag: Cambridge University Press
• Erscheinungstermin: 16. November 2009
• Englisch
• ISBN-13: 9780511654589
• Artikelnr.: 38215490

Inhaltsangabe

Part I. Basic Technologies: 1. Real time PCR Mickey Williams; 2.
Thermostable enzymes used in PCR Sudip K. Rakshit; 3. Inventing molecular
beacons Fred Russell Kramer, Salvatore A. E. Marras and Sanjay Tyagi; 4.
Rapid PCR and melting analysis Carl T. Wittwer, Randy P. Rasmussen and Kirk
M. Ririe; 5. PCR and fluorescence chemistries: DNA incarnate Ben Sowers; 6.
Analysis of microRNA expression by qPCR Vladimir Benes, Jens Stolte, David
Ibberson, Mirco Castoldi and Martina Muckenthaler; 7. Miniaturized PCR for
quantitative clinical diagnostics Melissa Mariani, Lin Chen and Philip J.
Day; 8. The road from qualitative to quantitative assay: what is next?
Michael W. Pfaffl; 9. Taking control of PCR Tania Nolan, Tanya Novak and
Jim Huggett; Part II. Applications: 10. PCR-based methods for the detection
of cancer cells in blood, bone marrow and lymph nodes for clinical
diagnostic assays Susan A. Burchill; 11. PCR and infectious diseases Jim
Huggett; 12. PCR and respiratory viruses Ian Mackay; 13. PCR and Severe
Acute Respiratory Syndrome (SARS) Yang Huanming and Weijun Chen; 14. The
MMR vaccine, measles virus, and autism: a cautionary tale Stephen A.
Bustin; 15. Non-invasive prenatal diagnosis using cell-free fetal nucleic
acids in maternal plasma Y. M. Dennis Lo; 16. PCR-based analyses of nucleic
acids from archival material Ulrich Lehmann; 17. Microarrays and qPCR Elisa
Wurmbach; 18. PCR in the detection of genetic variation Pui-Yan Kwok; 19.
PCR: a blessing and a curse for ancient DNA research Michael Hofreiter and
Holger Rompler.