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Plant Cells and Their Organelles provides a comprehensive overview of the structure and function of plant organelles. The text focuses on subcellular organelles while also providing relevant background on plant cells, tissues and organs. Coverage of the latest methods of light and electron microscopy and modern biochemical procedures for the isolation and identification of organelles help to provide a thorough and up-to-date companion text to the field of plant cell and subcellular biology. The book is designed as an advanced text for upper-level undergraduate and graduate students with student-friendly diagrams and clear explanations.…mehr
Plant Cells and Their Organelles provides a comprehensive overview of the structure and function of plant organelles. The text focuses on subcellular organelles while also providing relevant background on plant cells, tissues and organs. Coverage of the latest methods of light and electron microscopy and modern biochemical procedures for the isolation and identification of organelles help to provide a thorough and up-to-date companion text to the field of plant cell and subcellular biology. The book is designed as an advanced text for upper-level undergraduate and graduate students with student-friendly diagrams and clear explanations.
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About the Editors William V. Dashek, Emeritus Professor, Mary Baldwin College, USA Dr. Dashek is the recipient of BS, MS and Ph.D. degree from Marquette University where he was a National Institute of Health pre-doctoral trainee. He was a post-doctorate fellow at the Michigan State University Plant research Laboratory. Subsequently, he began a lengthy Academic teaching and research career. Currently, Dashek is retired writing advanced textbooks. Gurbachan S. Miglani, Visiting Professor, School of Agricultural Biotechnology, Punjab Agricultural University, India Dr. Miglani obtained B.Sc. (Agri. & A.H.) and M.Sc. (Genetics) from Punjab Agricultural University (PAU), Ludhiana, Punjab, India and Ph.D. from Howard University, Washington, D.C. During his 35-year teaching stint at PAU he taught general and specialized courses in genetics. Even after retirement Miglani is still teaching molecular genetics and biotechnology at PAU. In addition to research and review papers, popular science articles, laboratory manuals he has published a dozen books and presently he is writing his next book.
Inhaltsangabe
Contributors ix
Preface xi
Acknowledgments xii
1 An introduction to cells and their organelles 1 William V. Dashek
Cells 1
Cell organelles - an introduction 6
Ion channels 10
Proton pumps 14
Water channels 14
Carriers 15
Cell death 17
References 18
Further reading 24
2 Isolation and characterization of subcellular organelles from plant cells 25 Milee Agarwal, P. Desai, and Harish Padh
Isolation of subcellular organelles 26
Identification and characterization of isolated organelles 33
Summary 39
References 39
Further reading 41
3 Endoplasmic reticulum 42 William V. Dashek
Structure 42
Chemical composition 42
Biogenesis 45
Functions 45
Posttranslational events 49
Inhibitors 53
In vitro protein synthesis 54
Other functions 54
References 54
Further reading 60
4 The Golgi apparatus 61 D. Davis, T.E. Wilkop, and Georgia Drakakaki
The Golgi apparatus 61
Plant Golgi introduction 66
Structure and organization 69
Golgi-mediated vesicular trafficking 71
Plant Golgi-dependent cellular processes 74
Imaging and visualization 76
Isolation and analysis 78
Golgi genetics and genomics 81
Significance 84
Acknowledgment 85
References 85
Further reading 87
5 Microbodies 88 Robert Donaldson
Introducing peroxisomes 88
Leaf peroxisomes 89
Peroxisomes in oil seeds and pollen 91
References 107
Further reading 109
6 Microtubules, intermediate filaments, and actin filaments 110 William V. Dashek
Microtubules 110
Intermediate filaments 113
Actin filaments (microfilaments) 116
References 119
Further reading 124
7 The mitochondrion 125 Ray J. Rose, Terence W.-Y. Tiew, and William V. Dashek
Structure and dynamics 125
The mitochondrial genome 128
Comparison of the mitochondrial genome with chloroplast and nuclear genomes 131
The mitochondrial proteome and protein import 132
Respiratory metabolite transporters 133
The electron transport chain and oxidative phosphorylation 133
The alternative electron transfer chain in plant mitochondria 139
Plant mitochondria, stress responses and programmed cell death 139
Other functions of plant mitochondria 140
References 144
Further reading 145
8 Nucleus 146 Yogesh Vikal and Dasmeet Kaur
Structural organization of the NE 147
Nuclear pores 152
The nucleolus 157
Chromatin and chromosomes 165
DNA structure 170
DNA replication 173
RNA structure, function, and synthesis 176
Nucleocytoplasmic transport, nuclear import, and nuclear export 183
The dynamics of NE biogenesis during mitosis 188
The dynamics of nuclear pore complex biogenesis 196
Cell cycle control 200
Summary 205
References 206
Further reading 207
9 Plant cell walls 209 James E. Bidlack and William V. Dashek
