Plant Cytogenetics (eBook, PDF)

Methods and Protocols
Redaktion: Kianian, Shahryar F.; Kianian, Penny M. Avoles

• Format: PDF

Produktbeschreibung

This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics.

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 198
• Erscheinungstermin: 10. August 2016
• Englisch
• ISBN-13: 9781493936229
• Artikelnr.: 64692076

Autorenporträt

Shahryar Kianian Research Leader USDA-ARS Cereal Disease Laboratory University of Minnesota St. Paul, MN 55108  Email: [e-mail] Penny M Avoles Kianian Alderman Hall, St. Paul, MN 55108   Email:[e-mail] 

Inhaltsangabe

C-Banding of Plant Chromosomes.- Chromosome Painting by GISH and Multi-ColorFISH.- Fluorescent In Situ Hybridization on Extended Chromatin Fibers forHigh-Resolution Analysis of Plant Chromosomes.- Tyramide Signal Amplification: FluorescenceIn Situ Hybridization for IdentifyingHomologous Chromosomes.- Localizationof Low-Copy DNA Sequences on Mitotic Chromosomes by FISH.- Immunolabelingand In Situ Labelling of IsolatedPlant Interphase Nuclei.- Manipulationof Homologous Chromosome Recombination in Wheat.- DissectingPlant Chromosomes by the Use of Ionizing Radiation.- OpticalNano-Mapping and Analysis of Plant Genomes.- FlowSorting Plant Chromosomes.- Constructionof BAC Libraries from Flow-Sorted Chromosomes.- TheChromosome Microdissection and Microcloning Technique.- Immunolocalizationon Whole Anther Chromosome Spreads for Male Meiosis.- MappingRecombination Initiation Sites Using Chromatin Immunoprecipitation.- ChromatinImmunoprecipitation to Study Plant Epigenome.