This manual covers the whole range of molecular biology techniques, genetic engineering as well as cytogenetics of plants. Each chapter starts with an introduction into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting. The first part of the book introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning. The second part describes analytical approaches, in particular RAPD and RFLP, while the final part encompasses a variety of gene transfer techniques and both molecular and cytological analysis. The manual will be of great use to both the first-timer and the experienced scientist.
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"Overall, this manual deserves to be highly recommended due, in particular, to the clarity of writing. ...this manual will be purchased and used by many in this rapidly expanding area of research." Heredity
"...presents a broad range of techniques needed by plant molecular biologists. The protocols are clearly presented and referenced." The New Phytologist
"...presents a broad range of techniques needed by plant molecular biologists. The protocols are clearly presented and referenced." The New Phytologist