Plant Stress Tolerance (eBook, PDF)

Methods and Protocols
Redaktion: Sunkar, Ramanjulu

• Format: PDF

Produktbeschreibung

This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase-seq for identifying nucleosome occupancy, alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.

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Produktdetails

• Verlag: Springer New York
• Seitenzahl: 293
• Erscheinungstermin: 13. Juni 2024
• Englisch
• ISBN-13: 9781071639733
• Artikelnr.: 72240514

Inhaltsangabe

Strategies of Molecular Signal Integration for Optimized Plant Acclimation to Stress Combinations.- MNase-Seq Analysis for Identifying Stress-Altered Nucleosome Occupancy in Plants.- MeRIP-Seq for Identifying Stress-Responsive Transcriptome-Wide m6A Profiles in Plants.- Isolation and Visualization of Plant Stress Granule-Associated Components via On-Beads Digestion and Co-Localization Analysis.- Determination of Differential Alternative Splicing Under Stress Conditions.- A Method to Quantitatively Examine Heat Stress-Induced Alternative Splicing in Plants by RNA-Seq and RT-PCR.- Thiol Redox Proteomics for Identifying Redox-Sensitive Cysteine Residues Within the Protein of Interest During Stress.- Detection of Stress-Induced Changes in Subcellular Protein-Distribution.- Validation of the Interaction Between Stress-Responsive LncRNAs and RNA-Binding Proteins In Vitro.- Measurement of Photorespiratory Cycle Enzyme Activities in Leaves Exposed to Abiotic Stress.- In Vitro Kinase Assay with Recombinant SnRK2s: An Example for Assaying Stress-Responsive Kinases in Plants.- Identification of Stress-Responsive Metabolites in Plants Using an Untargeted Metabolomics Approach.- Measurement of Reactive Oxygen Species and Nitric Oxide from Tomato Plants in Response to Abiotic and Biotic Stresses.- Reactive Oxygen Species (ROS) Measurement in Arabidopsis Guard Cells in Response to Biotic and Abiotic Stresses.- Detection of Reactive Oxygen Species in Plant Root Immunity.- Studying Thermopriming-Mediated Short- and Long-Term Acquired Thermotolerance in Arabidopsis thaliana.- X-Ray Film Contact Print Method for Analyzing Differential Expression of Plant Protease Inhibitors Under Abiotic Stress.- Isolation and Identification of Growth-Promoting Bacteria from Plants Growing Under Abiotic Stresses.- Functional Characterization and Localization of Plant-Growth Promoting Bacteria Grown Under Stressful Conditions.- Generating Mutant for a Stress-Responsive Gene in Rice Using CRISPR-Cas9 System.