Platelets and Megakaryocytes (eBook, PDF)

Volume 1: Functional Assays
Redaktion: Gibbins, Jonathan M.; Mahaut-Smith, Martyn P.

• Format: PDF

Produktbeschreibung

Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 1: Functional Assays, leading expert researchers-many responsible for seminal discoveries in the field-describe basic and advanced techniques for analyzing platelet and megakaryocyte function in step-by-step detail. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. Techniques presented for megakaryocyte function research include those used to study the development of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. All protocols follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques offers a state-of-the-art survey of our understanding of these cells, with emphasis on the role of cell signaling in their regulation and function, as well as the use of molecular and post-genomic techniques for their study. Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 385
• Erscheinungstermin: 4. Februar 2008
• Englisch
• ISBN-13: 9781592597826
• Artikelnr.: 64692108

Inhaltsangabe

Platelet Functional Assays.- Effects of Anticoagulants Used During Blood Collection on Human Platelet Function.- Preparation of Washed Platelet Suspensions From Human and Rodent Blood.- Platelet Counting.- Electron Microscopy Methods for Studying Platelet Structure and Function.- Platelet Aggregation.- Platelet Aggregation in Whole Blood.- Secretion From Dense Granules.- Platelet Dense-Granule Secretion.- Analysis of the Releasable Nucleotides of Platelets.- Studies of Secretion Using Permeabilized Platelets.- Measurement of Platelet Arachidonic Acid Metabolism.- Measurement of the Platelet Procoagulant Response.- Platelet Adhesion Assays Performed Under Static Conditions.- Platelet Adhesion Assays Under Flow Using Matrix Protein-Coupled Adhesion Columns.- Techniques to Examine Platelet Adhesive Interactions Under Flow.- In Vivo Models of Platelet Function and Thrombosis.- Adhesion Between Platelets and Leukocytes or Endothelial Cells.- In Vitro Measurement of High-Shear Platelet Adhesion and Aggregation by the PFA-100®.- Flow-Cytometric Analysis of Platelet-Membrane Glycoprotein Expression and Platelet Activation.- Flow-Cytometric Analysis of Mouse Platelet Function.- Platelet Microparticles.- Megakaryocyte Functional Assays.- Isolation of Primary Megakaryocytes and Studies of Proplatelet Formation.- Isolation and Culture of Megakaryocyte Precursors.- Assays of Megakaryocyte Development.- Assays of Megakaryocyte Development.- Thrombopoietin Bioassay.- Culture of Megakaryocytic Cell Lines.

Rezensionen

"...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes." - Veterinary Pathology