Platelets and Megakaryocytes (eBook, PDF)

Volume 2: Perspectives and Techniques
Redaktion: Gibbins, Jonathan M.; Mahaut-Smith, Martyn P.

• Format: PDF

Produktbeschreibung

Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques leading expert researchers-many responsible for seminal discoveries in the field-offer basic and advanced techniques for studying cell signaling in the regulation and function of platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts in the regulation of cell signaling, and the measurement of intracellular calcium levels. There are also techniques for the study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. The application of many molecular and post-genomic methods to the study of platelets and megakaryocytes is also described. All protocols follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 1: Functional Assays describes in step-by-step detail basic and advanced techniques for analyzing platelet and megakaryocyte function. Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 504
• Erscheinungstermin: 2. Februar 2008
• Englisch
• ISBN-13: 9781592597833
• Artikelnr.: 64692498

Inhaltsangabe

Perspectives.- Signaling Receptors on Platelets and Megakaryocytes.- Manipulation of Gene Expression in Megakaryocytes.- In Vitro Changes of Platelet Parameters.- Pharmacological Approaches to Studying Platelet Function.- Inhibitors of Cellular Signaling Targets.- Signaling Techniques.- Ligand-Binding Assays for Collagen.- Use of Radiolabeled 2-Methylthio-ADP to Study P2Y Receptors on Platelets and Cell Lines.- Ligand-Binding Assays.- Techniques for Analysis of Proteins by SDS-Polyacrylamide Gel Electrophoresis and Western Blotting.- Study of Tyrosine Kinases and Protein Tyrosine Phosphorylation.- Protein Tyrosine Phosphatases.- The Study of Serine-Threonine Kinases.- Phosphoinositides.- Isolation and Analysis of Platelet Lipid Rafts.- Measurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell Cultures.- Measurement and Manipulation of Intracellular Ca2+ in Single Platelets and Megakaryocytes.- Patch-Clamp Recordings of Electrophysiological Events in the Platelet and Megakaryocyte.- Evaluation of Nitrotyrosine-Containing Proteins in Blood Platelets.- Nitric Oxide Signaling in Platelets.- Snake Venom Toxins Affecting Platelet Function.- Peptide Synthesis in the Study of Collagen-Platelet Interactions.- Platelet Permeabilization.- Molecular and Post-Genomic Techniques.- Using Retroviruses to Express Genes in Primary Megakaryocyte Lineage Cells.- Use of Antisense Oligonucleotide Technology to Investigate Signaling Pathways in Megakaryocytes.- GFP Fusion Proteins to Study Signaling in Live Cells.- Two-Dimensional Polyacrylamide Gel Electrophoresis for Platelet Proteomics.- Preparation of mRNA and cDNA Libraries From Platelets and Megakaryocytes.- Platelet Receptor Structures and Polymorphisms.- Gene Array Technology and the Study of Platelets and Megakaryocytes.

Rezensionen

"...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes." - Veterinary Pathology