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Inhaltsangabe
The Retroviral Life Cycle and the Molecular Construction of Retrovirus Vectors.- Construction of Retro viral Packaging Cell Lines.- Making High Titer Retroviral Producer Cells.- Methods of Titrating Nonselectable Recombinant Retroviruses.- Selectable Markers for Eukaryotic Cells.- Virus Genome Detection by the Polymerase Chain Reaction (PCR).- Syncytial Assays.- Neutralization of Virus.- Vesicular Stomatitis Virus Pseudotypes of Retroviruses.- Cell Lineage Studies Using Retroviruses.- Retroviral Vectors as Insertional Mutagens.- Assessment of Compounds for Anti-HIV Activity.- Analysis of Protein Factors that Interact with Adenovirus Early Promoters.- COS Cell Expression.- Papillomaviruses and Assays for Transforming Genes.- Herpes Simplex Virus Life Cycle and the Design of Viral Vectors.- Growth of Herpes Simplex Virus and Purification of Viral DNA.- Vectors Based on Epstein-Barr Virus.- Immortalization of Human B-Lymphocytes by Epstein-Barr Virus.- Vaccinia Virus as an Expression Vector.- Isolation and Handling of Recombinant Vaccinia Viruses.- Design and Construction of Poliovirus Epitope Expression Vectors.- Growth and Characterization of Poliovirus Antigen Chimeras.- Expression Vectors for the Construction of Hybrid Ty-VLPs.- Production and Purification of Hybrid Ty-VLPs.- Baculovirus Expression Vectors.- Baculovirus Expression Vectors.- Baculovirus Expression Vectors.
The Retroviral Life Cycle and the Molecular Construction of Retrovirus Vectors.- Construction of Retro viral Packaging Cell Lines.- Making High Titer Retroviral Producer Cells.- Methods of Titrating Nonselectable Recombinant Retroviruses.- Selectable Markers for Eukaryotic Cells.- Virus Genome Detection by the Polymerase Chain Reaction (PCR).- Syncytial Assays.- Neutralization of Virus.- Vesicular Stomatitis Virus Pseudotypes of Retroviruses.- Cell Lineage Studies Using Retroviruses.- Retroviral Vectors as Insertional Mutagens.- Assessment of Compounds for Anti-HIV Activity.- Analysis of Protein Factors that Interact with Adenovirus Early Promoters.- COS Cell Expression.- Papillomaviruses and Assays for Transforming Genes.- Herpes Simplex Virus Life Cycle and the Design of Viral Vectors.- Growth of Herpes Simplex Virus and Purification of Viral DNA.- Vectors Based on Epstein-Barr Virus.- Immortalization of Human B-Lymphocytes by Epstein-Barr Virus.- Vaccinia Virus as an Expression Vector.- Isolation and Handling of Recombinant Vaccinia Viruses.- Design and Construction of Poliovirus Epitope Expression Vectors.- Growth and Characterization of Poliovirus Antigen Chimeras.- Expression Vectors for the Construction of Hybrid Ty-VLPs.- Production and Purification of Hybrid Ty-VLPs.- Baculovirus Expression Vectors.- Baculovirus Expression Vectors.- Baculovirus Expression Vectors.
Rezensionen
I found the writing style uniformly clear and straightforward throughout....I noticed the well-organized, parallel structure of most chapters, which made them easy to follow and gave evidence of some rather careful editing. Practical Molecular Virology provides a concise, well-organized, well-written...selective overview of eukaryotic viral vectors. As an entry-level text, it surely will assist those wishing to use such vectors to make a choice among established systems. Does this book meet its objective? I believe it does. Methods books are notoriously hard to execute well, but this one succeeds by carefully defining its own niche and then occupying it. -ASM News
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