PRINS and In Situ PCR Protocols (eBook, PDF)

Redaktion: Pellestor, Franck

• Format: PDF

Produktbeschreibung

Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 244
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781597450683
• Artikelnr.: 64700714

Inhaltsangabe

Part I. Basic Methodology
New Rapid Multicolor PRINS Protocol
Ju Yan, Macoura Gadji, Kada Krabchi, and Régen Drouin
PRINS Combined With Peptide Nucleic Acid Labeling
Franck Pellestor and Petra Paulasova
PRINS Combined With Indirect Immunofluorescence
Philippe Coullin
PRINS for the Detection of Unique Sequences
Stephen S. Wachtel and Avirachan T. Tharapel
Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS)
Jørn Koch
Analysis of Sperm Aneuploidy by PRINS
Franck Pellestor, Brigitte Andréo, Jacques Puechberty, Geneviève Lefort, and Pierre Sarda
In Situ PCR Protocols
Omar Bagasra and Twaina Harris
Part II. Research and Clinical Applications
Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS
Jørn Koch
PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei
Antonella Russo
PRINS for the Detection of Gene Deletions in Cancer
Avirachan T. Tharapel and Stephen S. Wachtel
Combined Fluorescence In Situ Hybridization and PRINS for the Analysis of the Dystrophin Gene
Liborio Stuppia, Dario La Sala, and Caterina Cinti
Brain Tissue Preparations for Chromosomal PRINS Labeling
Ivan Y. Iourov, Svetlana G. Vorsanova, Franck Pellestor, and Yuri B. Yurov
PRINS on Plant Chromosomes
Jirí Macas, Alice Navrátilová, Marie Kubaláková, and Jaroslav Dolezel
Dual-Color PRINS for In Situ Detection of Fetal Cells in Maternal Blood
Kada Krabchi, Macoura Gadji, Ju Yan, and Régen Drouin
PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos
Franck Pellestor, Brigitte Andréo, Tal Anahory, and Samir Hamamah
Microdissection With PCR In Situ
Allen T. Christian and Christine Hara
Application of Reverse Transcription In Situ PCR in Cancer Analysis
Tamotsu Takeuchi, Yoshihiro Adachi, Hiroshi Sonobe, and Yuji Ohtsuki
In Situ Reverse Transcription PCR on Plant Tissues
Zbigniew Przybecki, Ewa Siedlecka, Marcin Filipecki, and Ewa Urbanczyk-Wochniak
In Situ PCR for the Detection of Human Cytomegalovirus in Suspension Cells During the Latent Phase of Infection
Barry Slobedman
Development and Adaptation of the PRINS Technology: An Overview
Franck Pellestor
Latest Developments in In Situ PCR
Omar Bagasra and Twaina Harris
Index

Rezensionen

From Reviews of the First Edition...

"...essential for any laboratory contemplating exploiting this powerful technique." -Microbiology Today

"...[a] splendid little book which covers the field quite well,,," -FEBS Letters

"...accessible and very practical...recommend[ed]...to any laboratory with an operational PCR machine...a valuable addition to the methods literature." -Quarterly Review of Biology

"...well written...contains sufficient detail for a novice to successfully complete the experiment...recommend[ed]...for libraries as well as individual researchers interested in applying PRINS technology." -Doody's Health Sciences Book Review Journal