Protein Phosphatase Protocols (eBook, PDF)

Redaktion: Ludlow, John W.

• Format: PDF

Produktbeschreibung

In Protein Phosphatase Protocols, John Ludlow assembles a collection of cutting-edge techniques for investigating the structure and function of protein serine/threonine phosphatases. These methods are designed to enable the investigator to isolate, identify, and assay phosphatase activities from both prokaryotic and eukaryotic sources. Written by leading researchers with extensive practice in their use, each method is time-tested and sufficiently detailed to ensure reproducibility for both new and established investigators. The book also includes a discussion of the functional significance of this group of enzymes with respect to cell growth and development. Protein Phosphatase Protocols offers researchers the single best collection of step-by-step laboratory protocols now available for these important enzymes. Because of their easy reproducibility, these methods will greatly facilitate research in the area, and thus are certain to become indispensable for all those working to unlock the structure and function of the protein serine/threonine phosphatases.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 316
• Erscheinungstermin: 2. Februar 2008
• Englisch
• ISBN-13: 9781592592678
• Artikelnr.: 64613846

Inhaltsangabe

Prokaryotic Protein-Serine/Threonine Phosphatases.- Protein Phosphatase Type 1 and Type 2A Assays.- Analyzing Gene Expression with the Use of Serine/Threonine Phosphatase Inhibitors.- Inhibitor-1, a Regulator of Protein Phosphatase 1 Function.- I1 PP2A and I2 PP2A.- Control of PP1 Activity Through Phosphorylation by Cyclin-Dependent Kinases.- Regulation of Neuronal PP1 and PP2A During Development.- PTPA Regulating PP2A as a Dual Specificity Phosphatase.- Microinjection and Immunological Methods in the Analysis of Type 1 and 2A Protein Phosphatases from Mammalian Cells.- Use of Immunocomplexed Substrate for Detecting PP1 Activity.- The Biochemical Identification and Characterization of New Species of Protein Phosphatase 1.- The Relationship Between Insulin Signaling and Protein Phosphatase 1 Activation.- Analysis of the Isoforms of Protein Phosphatase 1 (PP1) Isoforms with Polyclonal Peptide Antibodies.- Expression of Mouse Protein Phosphatase 2C in Eschericia coli and COS 7 Cells.- Expression of Functional Protein Phosphatase 1 Catalytic Subunit in E. coli.- Protein Phosphatase 2A and Protein Phosphatase X Genes in Arabidopsis thaliana.- Separation of Protein Phosphatase Type 2C Isozymes by Chromatography on Blue Sepharose.- Chromatographic Isolation of PP2A from Limulus Lateral Eyes.- Purification and Assay of the Ptc/Tpd1 Protein Phosphatase 2C from the Yeast Saccharomyces cerevisiae.- Molecular Cloning of Protein Phosphatase Type 2C Isoforms from Retinal cDNA.- Analysis of Protein Interactions Between Protein Phosphatase 1 and Noncatalytic Subunits Using the Yeast Two-Hybrid Assay.- Identifying Protein Phosphatase 2A Interacting Proteins Using the Yeast Two-Hybrid Method.- Protein Phosphatase 2A Regulatory Subunits.- Synthetic Lethal Screening in Protein PhosphatasePathways.- The Search for the Biological Function of Novel Yeast Ser/Thr Phosphatases.

Rezensionen

"I would highly recommend this volume for anyone wishing to develop or optimize Ser/Thr protein phosphatase assays and/or biochemical procedures in their laboratories."-The Quarterly Review of Biology