Protein Purification (eBook, ePUB)

Principles, High Resolution Methods, and Applications
Redaktion: Janson, Jan-Christer

• Format: ePub

Produktbeschreibung

The authoritative guide on protein purification--now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: * High-resolution reversed-phase liquid chromatography * Electrophoresis in gels * Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients * Affinity ligands from chemical and biological combinatorial libraries * Membrane separations * Refolding of inclusion body proteins from E. coli * Purification of PEGylated proteins * High throughput screening techniques in protein purification * The history of protein chromatography

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 544
• Erscheinungstermin: 3. Januar 2012
• Englisch
• ISBN-13: 9781118002193
• Artikelnr.: 37363935

Autorenporträt

JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.

Inhaltsangabe

Preface to The Third Edition vii
Preface to The Second Edition ix
Preface to The First Edition xi
Contributors xiii
Part I Introduction 1
1 Introduction to Protein Purification 3
Bo Ersson, Lars Rydén, and Jan-Christer Janson
Part II Chromatography 23
2 Introduction to Chromatography 25
Jan-Christer Janson and Jan Åke Jönsson
3 Gel Filtration: Size Exclusion Chromatography 51
Lars Hagel
4 Ion Exchange Chromatography 93
Evert Karlsson and Irwin Hirsh
5 High-Resolution Reversed-Phase Chromatography of Proteins 135
Sylvia Winkel Pettersson
6 Hydrophobic Interaction Chromatography 165
Kjell-Ove Eriksson and Makonnen Belew
7 Immobilized Metal Ion Affinity Chromatography 183
Lennart Kågedal
8 Covalent Chromatography 203
Francisco Batista-Viera, Lars Rydén, and Jan Carlsson
9 Affinity Chromatography 221
Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson
10 Affinity Ligands from Chemical Combinatorial Libraries 259
Enrique Carredano and Herbert Baumann
11 Affinity Ligands from Biological Combinatorial Libraries 269
Per- Åke Nygren
Part III Other Separation Methods and Related Techniques 279
12 Membrane Separations 281
Joachim K. Walter, Zuwei Jin, Maik W. Jornitz, and Uwe Gottschalk
13 Refolding of Inclusion Body Proteins from E. coli 319
Zhiguo Su, Diannan Lu, and Zheng Liu
14 Purification of PEGylated Proteins 339
Conan J. Fee and James M. Van Alstine
Part IV Electrophoresis 363
15 Electrophoresis in Gels 365
Reiner Westermeier
16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and
Immobilized pH Gradients 379
Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti
17 Two-Dimensional Electrophoresis in Proteomics 411
Reiner Westermeier and Angelika Görg
18 Protein Elution and Blotting Techniques 441
Reiner Westermeier
19 Capillary Electrophoretic Separations 451
Wolfgang Thormann
Part V Separation Method Optimization 487
20 High Throughput Screening Techniques in Protein Purification 489
Karol M. Lacki and Eggert Brekkan
Index 507