Protein Purification Protocols (eBook, PDF)

Redaktion: Cutler, Paul

• Format: PDF

Produktbeschreibung

In this new edition of the very successful Protein Purification Protocols (1996), Paul Cutler completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations. Comprehensive and highly practical, Protein Purification Protocols, Second Edition, brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and their functions today.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 484
• Erscheinungstermin: 2. Februar 2008
• Englisch
• ISBN-13: 9781592596553
• Artikelnr.: 64701908

Inhaltsangabe

General Strategies.- Preparation of Extracts From Animal Tissues.- Protein Extraction From Plant Tissues.- Extraction of Recombinant Protein From Bacteria.- Protein Extraction From Fungi.- Subcellular Fractionation of Animal Tissues.- Subcellular Fractionation of Plant Tissues.- The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds.- Avoidance of Proteolysis in Extracts.- Concentration of Extracts.- Making and Changing Buffers.- Purification and Concentration by Ultrafiltration.- Bulk Purification by Fractional precipitation.- Ion-Exchange Chromatography.- Hydrophobic Interaction Chromatography.- Affinity Chromatography.- Dye-Ligand Affinity Chromatography.- Lectin Affinity Chromatography.- Immunoaffinity Chromatography.- Immobilized Metal-Ion Affinity Chromatography.- Chromatography on Hydroxyapatite.- Thiophilic Affinity Chromatography and Related Methods.- Affinity Precipitation Methods.- Isoelectric Focusing.- Chromatofocusing.- Size-Exclusion Chromatography.- Fast Protein Liquid Chromatography.- Reversed-Phase Chromatography of Proteins.- Extraction of Membrane Proteins.- Removal of Detergent From Protein Fractions.- Purification of Membrane Proteins.- Lyophilization of Proteins.- Storage of Pure Proteins.- Electroelution of Proteins From Polyacrylamide Gels.- Electroblotting of Proteins From Polyacrylamide Gels.- Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses.- Microscale Solution Isoelectrofocusing.- Practical Column Chromatography.- Detection Methods.- Peptide Proteomics.- Multidimensional Liquid Chromatography of Proteins.- Mass Spectrometry.- Purification of Therapeutic Proteins.- Purification Process Scale-Up.

Rezensionen

From reviews of the first edition...
"...very useful..."
-Angewandte Chemie: International Edition

"...valuable to keep around the laboratory..."
-Biopharm

"...recommended for protein chemists....There is something in this book for
everyone....an excellent working book as well as an excellent reference."
-JAOCS: Journal of the American Oil Chemists' Society

"...an excellent lab companion..."
-SIM News

"...a worthwhile purchase for novice and established practitioners alike."
-Microbiology Today

"...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists."
-Biomedical Chromatography

"Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified protocols. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of protocols useful for both less and more experienced practitioners."-SGM Quarterly