Seema Anil Belorkar, Sudisha Jogaiah

Protocols and Applications in Enzymology (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses.

• Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards
• Presents easy to read, reproducible protocols for researchers and students across academia and industry
• Includes color diagrams that illustrate key concepts

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 266
• Erscheinungstermin: 19. November 2021
• Englisch
• ISBN-13: 9780323914376
• Artikelnr.: 63032488

Autorenporträt

Dr. Seema Anil Belorkar is Assistant Professor at the PG Department of Microbiology and Bioinformatics, Atal Bihari Vajpayee University, Bilaspur, with expertise in bioactive molecule production and enzymology. She has published 20 publications in various national and international journals, and has successfully completed 4 research projects with involvin production of enzymes and their application in agro-industrial wastes. Dr. Belorkar has contributed to 4 chapters in various books published by Springer, Wiley-Blackwell, and Elsevier. She has received 3 awards in the field of teaching and research, and has been a reviewer for articles in BMC Plant Biology, the Indian journal of Pharmaceutical Sciences and other journals of repute.Dr. Sudisha Jogaiah, Associate Professor and Head, Department of Environmental Science, Central University of Kerala. He is the Fellow of the National Academy of Agricultural Sciences (FNAAS), Fellow of National Academy of Biological Sciences (FNABS), Bridge Fellow, Government of Japan. He is the recipient of Sir C.V. Raman Young Scientist State Award by Chief Minister of Karnataka. He was awarded visiting Professorship by Yamaguchi University, Japan and Nanjing Agricultural University, China. He joined as Associate Professor in the Department of Environmental Science, Central University of Kerala in 2022. The technological quality and expertise of Dr. Jogaiah's lab is reflected in an extensive track record of publications with over 100 peer-reviewed papers with cumulative Thomson Reuters impact factor of 461.9, citations above 5053, one national and one International patent and 07 review articles with h-index - 41.

Inhaltsangabe

1. Introduction Enzymes-Past, present and Future
2. Distribution and diversity in microbial enzymes
3. Screening of potential microbes for enzymes of industrial significance
4. Solid state fermentations and SMF for enzyme production
5. Laboratory methods for enzyme assays
6. Structure and functions of enzyme kinetics
7. Protocols of important industrial enzymes
8. Strategies to improve enzyme activity for industrial processes
9. Scope and relevance of Industrial applications
10. Agro-industrial wastes for enzyme production
11. Significance of enzymes and their agricultural applications
12. Biotechnological applications of enzymes and future prospective
13. Conclusions