Public Health Microbiology (eBook, PDF)

Methods and Protocols
Redaktion: Spencer, John F. T.; Alicia L. Ragout De Spencer

• Format: PDF

Produktbeschreibung

The world abounds with microorganisms that are hazardous to human health in the normal course of life. In Public Health Microbiology: Methods and Protocols, expert investigators describe in step-by-step detail the laboratory techniques they have perfected for determining the nature and character of a diverse group of hazardous bacteria, viruses, and fungi. For bacterial and viral pathogens, the authors give special attention to PCR methods for detecting genes resistant to tetracycline, for resistance in Salmonella enterica, for identifying and typing Campylobacter coli, and for detecting the abundance of enteric, hepatitis A, and rotaviruses in sewage, as well as bacteriophages infecting the O157:H7 strain of Escherichia coli. For fungi the authors offer methods for computerized analysis and typing of fungal isolates, for the isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone. Other methods deal with hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, microbiological tests for sanitation equipment in factories, and the uses of animals in studies of various characteristics of lactobacilli. Review articles address the spread of pathogens from livestock and poultry production, including the threat of prion-based diseases. All protocols presented follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and highly practical, Public Health Microbiology: Methods and Protocols offers researchers and public health specialists alike a wide-ranging collection of methods for the determination of many important pathogenic microorganisms, their effects on health, and possible measures that can be taken to counter these effects.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 548
• Erscheinungstermin: 2. Februar 2008
• Englisch
• ISBN-13: 9781592597666
• Artikelnr.: 64700772

Inhaltsangabe

"Part I. Bacteria

Detection of Tetracycline Resistance Genes by PCR Methods
Rustam I. Aminov, Joanne C. Chee-Sanford, Natalie Garrigues, Asma Mehboob, and Roderick I. Mackie

Integron Analysis and Genetic Mapping of Antimicrobial Resistance Genes in Salmonella enterica serotype Typhimurium
Mairead Daly and Seamus Fanning

Molecular-Based Identification and Typing of Campylobacter jejuni and C. coli
Brigid Lucey, Fiona O'Halloran, and Seamus Fanning

Molecular Genotyping Methods and Computerized Analysis for the Study of Salmonella enterica
Ana Belen Vivanco, Juan Alvarez, Idoia Laconcha, Nuria López-Molina, Aitor Rementeria, and Javier Garaizar

Part II. Viruses

Detection of Infectious Rotaviruses by Flow Cytometry
Albert Bosch, Rosa M. Pintó, Jaume Comas, and Francesc-Xavier Abad

Integrated Cell Culture/PCR for Detection of Enteric Viruses in Environmental Samples
Kelly A. Reynolds

Abundance in Sewage of Bacteriophages Infecting Escherichia coli O157:H7
Maite Muniesa and Juan Jofre

Molecular Genotyping of Irish Rotavirus Strains
Fiona O'Halloran and Seamus Fanning

Hepatitis A Virus: Molecular Detection and Typing
Glòria Sßnchez, Cristina Villena, Albert Bosch, and Rosa M. Pintó

Part III. Fungi

Typing Fungal Isolates: Molecular Methods and Computerized Analysis
Aitor Rementeria, Ana Belen Vivanco, Ainara Cadaval, María Teresa Ruesga, Sonia Brena, Jose Pontón, Guillermo Quindós, and Javier Garaizar

Fungal Isolation and Enumeration in Foods
Dante Javier Bueno, Julio Oscar Silva, and Guillermo Oliver

Determination of Aflatoxins and Zearalenone in Different Culture Media
Dante Javier Bueno and Guillermo Oliver

Part IV. Other Pathogens

Intracellular Multiplication of Legionella Species and the Influence of Amoebae on Th eir Intracellular Growth "

Rezensionen

"...highly recommended for graduate students as well as advanced researchers working in the field of public health microbiology." - International Journal of Hygiene and Environmental Health