Quantitative Proteomics by Mass Spectrometry (eBook, PDF)

Redaktion: Sechi, Salvatore

• Format: PDF

Produktbeschreibung

This volume is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral. Attention is given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. This volume is an indispensable resource in the search for novel biomarkers.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 218
• Erscheinungstermin: 5. Februar 2008
• Englisch
• ISBN-13: 9781597452557
• Artikelnr.: 64702099

Inhaltsangabe

Acrylamide-A Cysteine Alkylating Reagent for Quantitative Proteomics.- Using Stable Isotope Tagging and Mass Spectrometry to Characterize Protein Complexes and to Detect Changes in Their Composition.- Stable Isotope Labeling by Amino Acids in Cell Culture for Quantitative Proteomics.- Quantitative Proteomics of Mouse Brain and Specific Protein-Interaction Studies Using Stable Isotope Labeling.- The Absolute Quantification Strategy.- Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling.- The Use of a Quantitative Cysteinyl-Peptide Enrichment Technology for High-Throughput Quantitative Proteomics.- An Isotope Coding Strategy for Proteomics Involving Both Amine and Carboxyl Group Labeling.- Proteolytic Labeling With 18O for Comparative Proteomics Studies.- Tandem Mass Spectrometry in the Detection of Inborn Errors of Metabolism for Newborn Screening.- Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope-Coded Affinity Tags.- Computational Analysis of Quantitative Proteomics Data Using Stable Isotope Labeling.- Quantitative Proteomic Analysis of Mammalian Organisms Using Metabolically Labeled Tissues.- Quantitative Proteomic Analysis of Phosphotyrosine-Mediated Cellular Signaling Networks.

Rezensionen

From the reviews:
"Quantitative Proteomics by Mass Spectrometry describes protocols of various quantitative proteomics procedures utilizing mass spectrometry. ... The publication of this book is timely. ... provides useful information regarding sample preparation/handling, reagent preparation/handling, mass spectrometry analysis etc. for successful experiments. ... In summary, the book serves as an excellent guide for both the experienced investigator and novice in the field who is contemplating using quantitative proteomics by mass spectrometry." (Masaru Miyagi, Journal of the American Society for Mass Spectrometry, Vol. 18, 2007)