Recombinant Proteins from Plants (eBook, PDF)

Redaktion: Cunningham, Charles; Porter, Andrew J. R.

• Format: PDF

Produktbeschreibung

In Recombinant Proteins from Plants, leading researchers from around the world present the latest molecular and classical approaches to the separation, analysis, and characterization of recombinant plant proteins. Focusing on the large-scale, cost-effective production of such proteins for use as specialist industrial or therapeutic biomolecules, these authoritative scientists also include promising experimental techniques that will become increasingly important in the future-such techniques as those for the efficient transformation of monocots with Agrobacterium tumefaciens, for optimizing the stability of recombinant proteins, and for exploiting the immunotherapeutic potential of plant-expressed proteins. Recombinant Proteins from Plants provides a comprehensive and detailed handbook of essential techniques that will benefit both scientists new to the field and established laboratories. It will become the standard reference for even highly skilled researchers working in this most exciting and fast developing field of biology.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 308
• Erscheinungstermin: 5. Februar 2008
• Englisch
• ISBN-13: 9781603272605
• Artikelnr.: 69699036

Inhaltsangabe

Rice Transformation by Agrobacterium Infection.- Barley as a Producer of Heterologous Protein.- Introducing and Expressing Genes in Legumes.- Use of the GUS Reporter Gene.- Expression of Recombinant Proteinase Inhibitors in Plants.- Detection of Recombinant Viral Coat Protein in Transgenic Plants.- Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants.- Tobamovirus Vectors for Expression of Recombinant Genes in Plants.- Single-Chain Fv Antibodies Expressed in Plants.- Characterization and Applications of Plant-Derived Recombinant Antibodies.- Production of Recombinant Antibodies in Plant Suspension Cultures.- Production of Foreign Proteins in Tobacco Cell Suspension Culture.- Transient Gene Expression in Plant Protoplasts.- Stability of Recombinant Proteins in Plants.- Trafficking and Stability of Heterologous Proteins in Transgenic Plants.- Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein.- Manipulation of Photosynthetic Metabolism.- Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA.- Analysis of N- and O-Glycosylation of Plant Proteins.- Two-Dimensional Electrophoresis.