Sample Preparation in Biological Mass Spectrometry (eBook, PDF)

Redaktion: Ivanov, Alexander R.; Lazarev, Alexander V.

• Format: PDF

Produktbeschreibung

The goal and philosophy of sample preparation in biological mass spectrometry is to reveal the actual multicomponent molecular structure of a biological specimen that can be assessed by mass spectrometry tools. Unfortunately, sample preparation is often looked at as a necessary but "boring" step which does not warrant any efforts beyond a paragraph or two in a "Methods" section; it is believed not to be conducive to innovation, and does not elicit the enthusiasm of funding agencies. Nevertheless, in recent years, the attention to sample preparation methods has risen considerably, both in Academia and in the life science industry. The aim of this book is to provide the researcher with important sample preparation strategies for a wide variety of analyte molecules, specimens, methods, and biological applications demanding mass spectrometric analysis as the detection end-point. In this volume we have compiled contributions from several laboratories that employ mass spectrometry for biological analysis.

With the latest inventions and the introduction of highly sophisticated mass spectrometry equipment, sample preparation has become an extremely important bottleneck of biomedical analysis. This book is structured as a compilation of contributed chapters ranging from step-by-step protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.

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Produktdetails

• Verlag: Springer Netherlands
• Seitenzahl: 1089
• Erscheinungstermin: 15. Juni 2011
• Englisch
• ISBN-13: 9789400708280
• Artikelnr.: 38385302

Inhaltsangabe

Introduction: Sample Preparation - Boring but Important! Section I. Traditional and improved techniques in sample preparation for proteomics.- Section II. Methods for Improved Proteolytic Digestion.- Section III. Methods for Tissue, Cell, and Organelle Preparation and Analysis.- Section IV. 2D Gel-based Proteomics.- Section V. Sample Preparation and Analysis Techniques for Biological Fluids and Biomarker Discovery.- Section VI. Sample Preparation Methods in Plant Proteomics Section.- VII. Affinity Interaction and Biochemical Enrichment Techniques.- Section VIII. Methods for Quantitative proteomics.- Section IX. Sample Preparation in Functional Proteomics.- Section X. Characterization of Membrane Proteins.- Section XI. Methods for MALDI MS Enabled Biomedical Applications.- Section XII. Clinical Research and Applications.- Section XIII. Sample Preparation Techniques in Metabolomics and Drug Discovery.- Section XIV. Sample Preparation in Analysis or Exotic and Limited Availability Specimens.- Section XV. Sample Treatment in Biodefense, Forensics and Infectious Diseases.- Section XVI. Novel Approaches in Sample Preparation and LC-MS Analysis.

Rezensionen

Anal Bioanal Chem (2012) 404:1331-1332: DOI 10.1007/s00216-012-6260-8
http://www.springerlink.com/content/7r2569j2175k81m2/

Alexander R. Ivanov, Alexander V. Lazarev (Eds): Sample preparation in biological mass spectrometry

by David C. Muddiman

"This book focuses on the burgeoning field of sample preparation as it relates to downstream integration with mass spectrometry. This is an expanding field, because of the diverse array of biological questions that can be addressed by use of mass spectrometry. A critical aspect of the successful outcome of an experiment is the 800-pound gorilla in the room called"sample preparation". It is widely accepted by the biological mass spectrometry community that sample preparation is a key aspect of effectively learning more about the in-depth biology under investigation."

"Sample Preparation in Biological Mass Spectrometry is a monograph which addresses a critical issue in a very diverse and important field. Several chapters are written by leaders in their field and are comprehensive and educational, and provide detailed procedures. Other chapters are less substantial in their content. Overall, the book has value for the novice getting started, because it provides a balance of background on the subject with some practical methods of analysis."