Second Harmonic Generation Imaging (eBook, ePUB)

Redaktion: Pavone, Francesco S.; Campagnola, Paul J.

• Format: ePub

Produktbeschreibung

Second-harmonic generation (SHG) serves as the basis for a microscope imaging contrast mechanism that visualizes cell and tissue structure and function. This book gives a complete overview of the principles underlying this technique and its current applications, including use in molecular sensing. Covering practical issues related to instrumentation and methods, the text offers detailed reviews of the capabilities and use of SHG in biomedical research, from imaging of cancer and skin disorders to self-assembled gels, holographic SHG imaging, and tissue engineering applications.

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Produktdetails

• Verlag: Taylor & Francis
• Erscheinungstermin: 19. April 2016
• Englisch
• ISBN-13: 9781040078730
• Artikelnr.: 72531537

Autorenporträt

Francesco S. Pavone is currently a full professor at the University of Florence in the Department of Physics and at the European Laboratory for Non-Linear Spectroscopy (LENS), and group leader at the Biophotonics Laboratories. His research group is involved in developing new microscopy techniques for high-resolution and high-sensitivity imaging, and laser manipulation purposes. He is also engaged in tissue imaging research. He is the author of more than 100 peer-reviewed journal articles, has delivered more than 60 invited talks, and is on the editorial board of several journals. He coordinates various European projects and has organized several international congresses. Paul J. Campagnola is an associate professor in the Departments of Biomedical Engineering and Physics at the University of Wisconsin-Madison. His research focuses on the development of nonlinear optical spectroscopy and microscopy methods, with an emphasis on translational applications. These research efforts are directed at understanding cancer cell-ECM interactions in the tumor microenvironment as well as fabricating scaffolds for tissue regeneration. He has published more than 50 peer-reviewed journal articles and several review articles and book chapters, and has delivered more than 70 invited talks. He serves on the editorial board for the Journal of Biomedical Optics and on numerous NIH and NSF review panels.

Inhaltsangabe

Principles of SHG Imaging: Theoretical Framework for SHG Microscopy: A
Matrix-Based Approach. How to Build an SHG Apparatus. THG Microscopy of
Cells and Tissues: Contrast Mechanisms and Applications. SHG Microscopy and
Its Comparison with THG, CARS, and Multiphoton Excited Fluorescence
Imaging. Biomedical Imaging Using SHG: Molecular Structure and Order with
Second-Harmonic Generation Microscopy. 3D SHG Imaging and Analysis of
Fibrillar Collagen Organization. Second-Harmonic Generation Imaging of
Microtubules. SHG and Optical Clearing. Holographic SHG Imaging. Imaging
Membrane Potential with SHG. Applications of SHG: Second-Harmonic
Generation Imaging of Self-Assembled Collagen Gels. SHG and Multiphoton
Fluorescence Imaging of the Eye. Multiphoton Excitation Imaging of the
Arterial Vascular Bed. Combined SHG/THG Imaging. SHG Imaging of Collagen
and Application to Fibrosis Quantization. Quantitative Approaches for
Studying the Role of Collagen in Breast Cancer Invasion and Progression.
SHG in Tumors: Scattering and Polarization. SHG Imaging for Tissue
Engineering Applications. Index.