T Cell Protocols (eBook, PDF)

Redaktion: De Libero, Gennaro

• Format: PDF

Produktbeschreibung

With a wide variety of investigative approaches, T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls.

Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.

---

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Produktdetails

• Verlag: Humana Press
• Seitenzahl: 166
• Erscheinungstermin: 2. Dezember 2008
• Englisch
• ISBN-13: 9781603275279
• Artikelnr.: 64701863

Inhaltsangabe

Analysis of Frequency and Phenotype of Antigen-Specific T Cells.- B Cell Helper Assays.- transkingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing.- Flow Cytometry and Cell Activation.- Investigating T Cells by Polychromatic Flow Cytometry.- Generation of Human T Cell Clones.- Limiting Dilution Analysis of Antigen-Specific T Cells.- T Cell Epitope-Mapping by Cytokine Gene Expression Assay.- Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications.- Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE.- Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation.

Rezensionen

From the reviews of the second edition:

"A great collection of protocols, some totally new and some upgraded ... to study the biology of this fascinating cell is wellcome by the scientific community. ... Thanks to the generation of ... PFC, is now at the hands of those scientists that want to advance our understanding of T cells biology. ... the book will be of enourmous help both to beginners and to experienced scientists is well underlining the esprit of the book, as I showed with the three examples illustrated." (CarloAlberto Redi, European Journal of Histochemistry, October, 2009)