Khursheed Hussain, Sameena Maqbool Lone, Khalid Z. Masoodi, Syed Minu Balkhi

Techniques for Biochemical Analysis (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids and more. The large range of protocols covered in this foundational how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.

• Features step-by-step methods for biochemical analysis of a variety of compounds
• Explores methods that are applicable and adaptable across a variety of fields, including biochemistry, molecular biology, and related areas
• Provides detailed instructions on how to prepare buffers, equipment to be used and analysis of a variety of molecules, including carbohydrates, lipids, proteins, and hormones
• Contains interdisciplinary and adaptable methods and techniques

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 270
• Erscheinungstermin: 4. April 2024
• Englisch
• ISBN-13: 9780443159152
• Artikelnr.: 70299934

Autorenporträt

Khursheed Hussain, has been serving as Assistant Professor at Sher-e-Kashmir University of Sciences & Technology in India for 13 years. He served as Scientific Officer to Vice Chancellor from 2015-2020. He has published more than 120 research articles, 25 review articles and many books. His contribution in the field of research is in varietal and hybrid development as Principal/Co-breeder of 7 vegetable varieties with other varieties in the pipeline. He has been an advisor of more than 50 PG students and has been invited to give many lectures and trainings.

Inhaltsangabe

1. Introduction to
2. Introduction to laboratory procedures
3. Preparation of solutions and buffers
4. Qualitative analysis of carbohydrates
5. Qualitative analysis of proteins
6. pH (negative logarithm of hydrogen ion concentration)
7. Spectrophotometry
8. Carbohydrates
9. Amino acids and proteins
10. Enzyme assays
11. Vitamin assays
12. Phenolics
13. Plant pigments
14. Plant
15. Antinutritional factors in plants
16. Toxins
17. Extraction and identification of polyacetylenes
18. Alkaloids
19. Plant hormones
20. Immunochemical methods
21. Nitrogenase activity
22. Chlorophyll
23. Proximate analysis
24. Minerals
25. Antinutritional factors in plants
26. Antioxidants
27. Lipids