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This book presents the fundamental principles and latest advances in the field, including the associated development of imaging techniques that exploit fluorescent proteins. The book first traces the history of fluorescent proteins and the revolution they enabled in cellular imaging. It then discusses fluorescent proteins with novel photophysical properties. The book also covers cutting-edge imaging applications, including variations of FRAP for assessing protein dynamics, FRET microscopy of living cells, and superresolution microscopies for molecular imaging.
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