The Giant Vesicle Book (eBook, PDF)

Redaktion: Dimova, Rumiana; Marques, Carlos

• Format: PDF

Produktbeschreibung

This practical manual gives the reader a toolbox for working with giant vesicles, including expert tips on preparation, measurement, and characterization methods. The contents build from a simple model to advanced membrane and cell system models. It also includes fundamentals for understanding vesicle structure, properties and behavior.

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Produktdetails

• Verlag: Taylor & Francis
• Seitenzahl: 676
• Erscheinungstermin: 19. November 2019
• Englisch
• ISBN-13: 9781498752183
• Artikelnr.: 58279037

Inhaltsangabe

Preparation methods for giant unilamellar vesicles. Preparation and properties of giant plasma membrane vesicles and giant unilamellar vesicles from natural membranes. Protein reconstitution in giant vesicles. GUVs with cytoskeleton. Understanding giant vesicles - a theoretical perspective. Simulating membranes, vesicles, and cells. Theory of vesicle dynamics in flow and electric fields. Particle-membrane interactions. Theory of polymer-membrane interactions. Application of optical microscopy techniques on giant unilamellar vesicles. Mechanics assays of synthetic lipid membranes based on micropipette aspiration. Atomic force microscopy of giant unilamellar vesicles. Manipulation and biophysical characterization of GUVs with an optical stretcher. Vesicle fluctuation analysis. Using electric fields to assess membrane material properties in GUVs. Creating membrane nanotubes from GUVs. Measuring GUV adhesion. Phase diagrams and tie lines in GUVs. Vesicle dynamics in flow: an experimental approach. Membrane permeability measurements. Lipid and protein mobility in GUVs. Shining light on membranes. Protein-membrane interactions. Effects of antimicrobial peptides and detergents on guvs. Lipid-polymer interactions: effect on GUVs shapes and behavior. Polymersomes. Giant hybrid polymer/lipid vesicles. Giant unilamellar vesicles: from protocell models to the construction of minimal cells. Encapsulation of aqueous two-phase systems and gels within giant lipid vesicles. Droplet-supported giant lipid vesicles as compartments for synthetic biology. List of lipids and physical constants of lipid bilayers. List of membrane dyes and fluorescent groups conjugated to lipids. List of detergents. List of water-soluble dyes or their fluorescent groups and their structures

Rezensionen

"Giant vesicles are artificial membrane systems used in basic biological research, applied research on smart materials, and medical and pharmaceutical applications. This massive volume provides a collection of 30 review articles, each an independent chapter, on this important topic. Editors Dimova (Max Planck Institute) and Marques (French National Centre for Scientific Research) are experts in the field, and the contributors represent an international group, with the majority of authors hailing from European countries. The chapters cover a diverse array of topics, including biophysics, cell and molecular biology, and physical chemistry. Because biophysics is a consideration, many chapters feature detailed mathematical treatments. The early part of the book provides a good summary of the field and its fundamental concepts. The articles are well referenced, drawing from the primary literature, and most include citations up to 2018. The book is beautifully illustrated with nice figures and callout boxes that highlight important protocols-some parts of the book resemble an advanced textbook. Chapter 4 (on the cytoskeleton) and chapter 10 (on light microscopy) were particularly informative to this reviewer. Chapters 15 and 19 provide web links to videos illustrating important concepts in vesicle dynamics. Several appendixes on topics such as membrane dyes and detergents are included."
-J. Z. Kiss, UNC-Greensboro, in CHOICE November 2020