The Hepatocyte Review (eBook, PDF)

Redaktion: Berry, M. N.; Edwards, Anthony M.

• Format: PDF

Produktbeschreibung

It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics.

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Produktdetails

• Verlag: Springer Netherlands
• Seitenzahl: 606
• Erscheinungstermin: 29. Juni 2013
• Englisch
• ISBN-13: 9789401733458
• Artikelnr.: 44178425

Inhaltsangabe

1. Methods and Biology.- I. Isolated hepatocytes: forty years on.- 2. Isolation and culture of human hepatocytes.- 3. Isolation and culture of porcine hepatocytes.- 4. Preparation and properties of isolated hepatocytes from sheep.- 5. Elasmobranch hepatocytes.- 6. Isolation and cultivation of teleost hepatocytes.- 7. Conditions for growth of hepatocytes in culture.- 8. Engineering of a miniature 3-D hepatic cell plate culture model.- 9. Freezing and hypothermic storage of porcine hepatocytes.- 10. Storage of isolated hepatocytes.- 11. Hepatocyte swelling: techniques and effects on metabolism.- 12. Permeabilisation of hepatocytes with ?-toxin.- 13. Perifused immobilised hepatocytes for metabolic studies.- 14. Small hepatocytes in primary cultures.- 2. Reviews of Hepatocyte Biology.- 15. Mechanisms of hepatocyte growth regulation by hormones and growth factors.- 16. Proliferative response and metabolic effects of growth factors in human hepatocytes.- 17. Growth control and cell cycle progression in cultured hepatocytes.- 18. Mechanisms of active cell death in isolated hepatocytes.- 19. The protective role of nitric oxide in hepatocytes during responses to inflammatory mediators and induction of apoptosis.- 20. Short-term regulation by insulin of glucose metabolism in isolated and cultured hepatocytes.- 21. Studies on mitochondrial-cytoplasmic interactions in isolated hepatocytes from fasted rats.- 3. Applications: Pharmacology and Toxicology.- 22. The importance of hepatocytes in drug metabolism studies: an industrial perspective.- 23. Human hepatocytes as an experimental system for the evaluation of xenobiotics.- 24. Constitutive expression of cytochromes P450 in rat hepatocyte culture.- 25. Optimising conditions for induction of cytochrome P450 in primary hepatocytecultures.- 26. The use of isolated hepatocytes to study the mechanisms of action of environmental contaminants.- 27. The use of isolated hepatocytes for the study of xenobiotic hepatotoxicity.- 4. Therapeutic Applications.- 28. Human hepatocyte transplantation: biology and therapy.- 29. Ex vivo hepatocyte-directed gene therapy.- 30. A review of bioartificial liver development from an artificial organ engineering perspective.- 31. Development and testing of a bioartificial liver.- 32. The Berlin extracorporeal liver support system.- 33. The bioartificial liver of the Academic Medical Center at Amsterdam.- 34. Performance of a flat membrane bioreactor utilizing porcine hepatocytes cultured in an extracellular matrix.