The Mitotic Spindle (eBook, PDF)

Methods and Protocols
Redaktion: Chang, Paul; Ohi, Ryoma

• Format: PDF

Produktbeschreibung

This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls. Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field.

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 424
• Erscheinungstermin: 19. Mai 2016
• Englisch
• ISBN-13: 9781493935420
• Artikelnr.: 64701906

Inhaltsangabe

Using FluorescenceMicroscopy to Study Mitosis.- Using PhotoactivatableGFP to Study Microtubule Dynamics and Chromosome Segregation.- Purification and Fluorescent Labelingof Tubulin from Xenopus Laevis Egg Extracts.- Measuringthe Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro.- Imagingand Quantifying the Dynamics of Gamma-Tubulin at Microtubule Minus Ends in Mitotic Spindles.- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy.- Encapsulationof Xenopus Egg and Embryo Extract SpindleAssembly Reactions in Synthetic Cell-Like Compartmentswith Tunable Size.- In Vitro Kinetochore Assembly.- Biochemicaland Structural Analysis of Kinetochore Histone-Fold Complexes.- Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells.- StudyingKinetochores In Vivo Using FLIM-FRET.- Purification of Fluorescently LabeledSaccharomyces Cerevisiae Spindle PoleBodies.- A Cell-FreeSystem for Real-Time Analyses of Centriole Disengagement and Centriole to CentrosomeConversion.- Assaysto Study Mitotic Centrosome and Spindle Pole Assembly and Regulation.- AnalyzingSpindle Positioning Dynamics in Cultured Cells.- Quantification of Mitotic Chromosome Alignment.- Imaging Mitosisin the Moss Physcomitrella Patens.- Small MoleculeApproach to Study the Function of Mitotic Kinesins.- Identificationand Characterization of Mitotic Spindle-Localized Transcripts.- Probing Mitosisby Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-InducibleDimerization.- StudyingKinetochore Kinases.- Engineeringand Functional Analysis of Mitotic Kinases through Chemical Genetics.- Using CellCulture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer.- Generationand Purification of Tetraploid Cells.- Anti-MicrotubuleDrugs.