Total Chemical Synthesis of Proteins (eBook, PDF)

Redaktion: Brik, Ashraf; Liu, Lei; Dawson, Philip

• Format: PDF

Produktbeschreibung

How to synthesize native and modified proteins in the test tube

With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins.

Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource:

• Contains valuable insights from the pioneers in the field of chemical protein synthesis
• Presents proven synthetic approaches for a range of protein families
• Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics

Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.

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Produktdetails

• Verlag: Wiley-VCH
• Erscheinungstermin: 23. Februar 2021
• Englisch
• ISBN-13: 9783527823574
• Artikelnr.: 61253651

Autorenporträt

Ashraf Brik, PhD, is a Professor of Chemistry at the Schulich Faculty of Chemistry in the Technion-Israel Institute of Technology. Philip E. Dawson, PhD, is a Professor of Chemistry at Scripps Research, CA, where he is also the Dean of Graduate and Postdoctoral Studies. Lei Liu, PhD, is a Professor of Chemistry at Tsinghua University.

Inhaltsangabe

Characterization of protein molecules prepared by total chemical synthesis
Automated fast flow peptide synthesis
N,S- and N,Se-acyl transfer devices in protein synthesis
Chemical synthesis of proteins through native chemical ligation of peptide hydrazides
Expanding native chemical ligation methodology with synthetic amino acid derivatives
Peptide ligations at sterically demanding sites
Controlling segment solubility in large protein synthesis
Towards HPLC-free total chemical synthesis of proteins
Solid phase chemical ligation
Ser/Thr ligation for protein chemical synthesis
Protein semisynthesis
Bio-orthogonal imine chemistry in chemical protein synthesis
Deciphering protein folding using chemical protein synthesis
Chemical synthesis of ubiquitinated proteins for biochemical studies
Glycoprotein synthesis
Chemical Synthesis of membrane proteins
Chemical synthesis of selenoproteins
Histone synthesis
Application of chemical synthesis to engineer protein backbone connectivity
Beyond phosphate esters - synthesis of unusually phosphorylated peptides and proteins for proteomic research
Cyclic peptides via ligation methods