Transgenic Wheat, Barley and Oats (eBook, PDF)

Production and Characterization Protocols
Redaktion: Jones, Huw D.; Shewry, Peter R.

• Format: PDF

Produktbeschreibung

The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. The in-depth chapters adhere to the highly successful Methods in Molecular Biology(TM) series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 349
• Erscheinungstermin: 14. November 2008
• Englisch
• ISBN-13: 9781597453790
• Artikelnr.: 64700510

Inhaltsangabe

Transgenic Wheat, Barley and Oats: Production and Characterization.- Transformation and Regeneration.- Selection of Transformed Plants.- Reporter Genes.- Biolistics Transformation of Wheat.- Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos.- Floral Transformation of Wheat.- Highly Efficient Agrobacterium-Mediated Transformation of Wheat Via In Planta Inoculation.- Barley Transformation Using Biolistic Techniques.- Barley Transformation Using Agrobacterium-Mediated Techniques.- Transformation of Oats and Its Application to Improving Osmotic Stress Tolerance.- Gene and Protein Expression.- Promoter Sequences for Defining Transgene Expression.- Down-Regulation of Gene Expression by RNA-Induced Gene Silencing.- Characterisation of Transgenic Plants.- Gene Insertion Patterns and Sites.- Fluorescent In Situ Hybridization to Detect Transgene Integration into Plant Genomes.- Establishing Substantial Equivalence: Transcriptomics.- Establishing Substantial Equivalence: Proteomics.- Establishing Substantial Equivalence: Metabolomics.- Design and Management of Field Trials of Transgenic Cereals.- GM Risk Assessment.- Conclusions.- Transgenic Wheat, Barley and Oats: Future Prospects.