UHPLC in Life Sciences (eBook, PDF)

Redaktion: Guillarme, Davy; Veuthey, Jean-Luc

• Format: PDF

Produktbeschreibung

Since its commercial introduction in 2004, UHPLC (Ultra-High Performance Liquid Chromatography) has begun to replace conventional HPLC in academia and industry and interest in this technique continues to grow. Both the increases in speed and resolution make this an attractive method; particularly to the life sciences and more than 1500 papers have been written on this strongly-evolving topic to date.

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Produktdetails

• Verlag: Royal Society of Chemistry
• Seitenzahl: 466
• Erscheinungstermin: 30. Juni 2012
• Englisch
• ISBN-13: 9781849735490
• Artikelnr.: 44763588

Autorenporträt

Davy Guillarme gained his PhD in analytical chemistry from the University of Lyon (France) in 2004. He is now lecturer at the School of Pharmaceutical Sciences, University of Geneva (Switzerland). He is working mainly on the development of new approaches to perform ultra-fast and high resolution separations in liquid chromatography. He is also interested in the coupling of these strategies with alternative detection modes, particularly mass spectrometry. Jean-Luc Veuthey obtained his PhD in analytical chemistry from the University of Geneva (Switzerland) in 1987. He is now full professor at the School of Pharmaceutical Sciences, University of Geneva (Switzerland). His interests include the development of LC and CE hyphenated to several detection modes for the analysis of drugs and metabolites. Sample preparation and validation of the procedures are also particularly studied in his laboratory.

Inhaltsangabe

1.1. Trends in HPLC
1.2. Comparison of Chromatographic Techniques and Supports
1.3. Constructing Kinetic Plots
1.4. History of the Kinetic Plot Method
1.5 Unification of the Isocratic and Gradient Kinetic Plot Method
1.6. Relation between the Kinetic Performance under Isocratic and Gradient Elution Conditions
1.7. Influence of the Test Conditions on the Obtained Kinetic-Performance Limit Curve
1.8. Some Reflections on Recent Trends in Liquid Chromatography using the Kinetic Plot Method
1.9. Conclusions
Acknowledgements
References
2.1 UHPLC Instrumentation
2.2 UHPLC Columns
3.1 Introduction
3.2 Qualitative Transfer from HPLC to UHPLC
3.3 Normative Context for the HPLC to UHPLC Transfer
3.4 Validation of UHPLC Methods and Equivalence of the HPLC-UHPLC Methods
3.5 Conclusions
3.6 References
4.1 Introduction
4.2 High throughput and high resolution in HT-UHPLC
4.3 Limitations of HTLC and HT-UHPLC
4.4 Advantage of high temperature in life science analysis
4.5 HT-UHPLC in comprehensive on-line two-dimensional liquid chromatography (LC x LC)
4.6 Conclusion
5. Comparison of the performance of totally porous and core-shell particles
5.1. Introduction
5.2. Column performance
5.3. Possibilities of recent core-shell technology
5.4. Particle size distribution and roughness of core-shell particles
5.5. Loading capacity of core-shell particles
5.6. Limited efficiency when core-shell particles packed in narrow-bore columns
5.7. Extra column effects, contribution to band broadening
5.8. Performance of core-shell and totally porous particles in isocratic elution mode
5.9. Performance of core-shell and totally porous particles in gradient elution mode
5.10. Conclusion
6.1 Introduction
6.2 Analytical Conditions for performing HILIC.
6.3 Applications of HILIC in UHPLC.
6.4 References.
7.1 Introduction
7.2 Selection of ionization techniques
7.3 Overview of mass analyzers and their main features
7.4 New developments in mass spectrometry applicable in UHPLC/MS
7.5 Conclusions
8.1 Introduction
8.2 Solubility
8.3 Ionization
8.4 Lipophilicity
8.5 Permeability
8.6 Conclusion
8.7 References
9.1 Introduction
9.2 UHPLC in Bioanalysis
9.3 Sample preparation for UHPLC in bioanalysis
9.4 Conclusions
10.1 Introduction
10.2 GC-MS Analyses
10.3 LC-MS(/MS) Analyses
10.4 Application of UHPLC-MS(/MS) for Drug Testing in Sports
10.5 Conclusion
References
11.1 Introduction
11.2 Use of UHPLC for the Analysis of Seized Drugs
12.1. Pharmaceuticals as environmental contaminants
12.2. Analysis of pharmaceuticals in environmental samples
12.3. Occurrence of pharmaceuticals in environmental and wastewater samples
12.4. Conclusions
Acknowledgements
References
13.1 Introduction
13.2 Multiple Facets of UHPLC in NP research
13.3 Fast Targeted Analysis
13.4 Fast Non-Targeted Analysis, Fingerprinting, and Metabolomics
13.5 High-Resolution Profiling and Metabolite ID
13.6 Conclusion
Acknowledgments
References
14.1. Introduction
14.2. Pre-analysis Considerations: Protocol Design, Sample Collection, Storage and Preparation
14.3. Sample Preparation for Serum and/Plasma
14.4. Collection and Storage of Urine Samples
14.5. UHPLC-MS-Based Metabolite Profiling
14.6. Applications of UHPLC-MS to Human Metabolic Profiling Studies
14.7 Current Challenges for UHPLC-MS in Global metabolic profiling Studies
14.8. Conclusions