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Radioligand binding in situ is the most sensitive method for detecting available receptors; the success and reliability of this approach are determined by a number of technical requirements and constraints. Based on the authors' fifteen years of experience in the field, Visualization of Receptors In Situ: Applications of Radioligand Binding explicitly details these procedures and provides step-by-step methods for visualizing receptors via radioligand binding - information not found in primary research materials. It covers many practical procedures, including radioligand and tissue preparations, in vitro assay, autoradiographic emulsion handling, and image analysis.
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