Tina M. Henkin, Joseph E. Peters

Snyder and Champness Molecular Genetics of Bacteria (eBook, PDF)

• Format: PDF

Produktbeschreibung

The single most comprehensive and authoritative textbook on bacterial molecular genetics Snyder & Champness Molecular Genetics of Bacteria is a new edition of a classic text, updated to address the massive advances in the field of bacterial molecular genetics and retitled as homage to the founding authors. In an era experiencing an avalanche of new genetic sequence information, this updated edition presents important experiments and advanced material relevant to current applications of molecular genetics, including conclusions from and applications of genomics; the relationships among recombination, replication, and repair and the importance of organizing sequences in DNA; the mechanisms of regulation of gene expression; the newest advances in bacterial cell biology; and the coordination of cellular processes during the bacterial cell cycle. The topics are integrated throughout with biochemical, genomic, and structural information, allowing readers to gain a deeper understanding of modern bacterial molecular genetics and its relationship to other fields of modern biology. Although the text is centered on the most-studied bacteria, Escherichia coli and Bacillus subtilis, many examples are drawn from other bacteria of experimental, medical, ecological, and biotechnological importance. The book's many useful features include * Text boxes to help students make connections to relevant topics related to other organisms, including humans * A summary of main points at the end of each chapter * Questions for discussion and independent thought * A list of suggested readings for background and further investigation in each chapter * Fully illustrated with detailed diagrams and photos in full color * A glossary of terms highlighted in the text While intended as an undergraduate or beginning graduate textbook, Molecular Genetics of Bacteria is an invaluable reference for anyone working in the fields of microbiology, genetics, biochemistry, bioengineering, medicine, molecular biology, and biotechnology. "This is a marvelous textbook that is completely up-to-date and comprehensive, but not overwhelming. The clear prose and excellent figures make it ideal for use in teaching bacterial molecular genetics." --Caroline Harwood, University of Washington Watch an interview with the authors as they discuss their book further: https://www.youtube.com/watch?v=NEl-dfatWUU

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 640
• Erscheinungstermin: 29. Juli 2020
• Englisch
• ISBN-13: 9781555819767
• Artikelnr.: 59905671

Autorenporträt

Tina M. Henkin is Professor of Microbiology and Robert W. and Estelle S. Bingham Professor of Biological Sciences at Ohio State University, where she has been teaching since 1995. Dr. Henkin received a PhD in genetics at the University of Wisconsin. Joseph E. Peters is Professor of Microbiology and Director of the Graduate Program in Microbiology at Cornell University, where he has been teaching since 2002. Dr. Peters received a PhD in microbiology at the University of Maryland.

Inhaltsangabe

Preface xv

Acknowledgments xix

About the Authors 1

Introduction 3

The Biological Universe 5

The Bacteria 5

The Archaea 7

The Eukaryotes 7

What is Genetics? 8

Bacterial Genetics 8

Bacteria Are Haploid 9

Short Generation Times 9

Asexual Reproduction 9

Colony Growth on Agar Plates 9

Colony Purification 9

Serial Dilutions 9

Selections 10

Storing Stocks of Bacterial Strains 10

Genetic Exchange 10

Phage Genetics 10

Phages Are Haploid 11

Selections

with Phages 11

Crosses with Phages 11

A Brief History of Bacterial Molecular Genetics 11

Inheritance in Bacteria 11

Transformation 11

Conjugation 12

Transduction 12

Recombination within Genes 12

Semiconservative DNA Replication 12

mRNA 12

The Genetic Code 12

The Operon Model 12

Enzymes for Molecular Biology 12

Synthetic Genomics 13

What is Ahead 13

1 The Bacterial Chromosome: DNA Structure, Replication, and Segregation 17

DNA Structure 17

The Deoxyribonucleotides 17

The DNA Chain 18

The 5' and 3' Ends 18

Base Pairing 20

Antiparallel Construction 20

The Major and Minor Grooves 21

The Mechanism of DNA Replication 21

Deoxyribonucleotide Precursor Synthesis 21

Replication of the Bacterial Chromosome 21

Replication of Double- Stranded DNA 26

Replication Errors 30

Editing 30

RNA Primers and Editing 31

Impediments to DNA Replication 31

Damaged DNA and DNA Polymerase III 31

Mechanisms To Deal with Impediments on Template DNA Strands 32

Physical Blocks to Replication Forks 32

Replication of the Bacterial Chromosome and Cell Division 32

Structure of Bacterial Chromosomes 34

Replication of the Bacterial Chromosome 34

Initiation of Chromosome Replication 34

RNA Priming of Initiation 35

Termination of Chromosome Replication 35

Chromosome Segregation 37

Coordination of Cell Division with Replication of the Chromosome 47

Timing of Initiation of Replication 49

The Bacterial Nucleoid 51

Supercoiling in the Nucleoid 51

Topoisomerases 52

The Bacterial Genome 55

Box 1.1 Structural Features of Bacterial Genomes 37

Box 1.2 Antibiotics That Affect Replication and DNA Structure 54

2 Bacterial Gene Expression: Transcription, Translation, Protein Folding, and Localization 61

Overview 61

The Structure and Function of RNA 62

Types of RNA 62

RNA Precursors 62

RNA Structure 62

RNA Processing and Modification 64

Transcription 64

Structure of Bacterial RNA Polymerase 64

Overview of Transcription 65

Details of Transcription 67

rRNAs and tRNAs 74

RNA Degradation 77

RNases 77

The Structure and Function of Proteins 78

Protein Structure 78

Translation 80

Structure of the Bacterial Ribosome 80

Overview of Translation 83

Details of Protein Synthesis 84

The Genetic Code 92

Polycistronic mRNA 96

Protein Folding and Degradation 98

Protein Chaperones 98

Protein Degradation 101

Protein Localization 101
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