Andere Kunden interessierten sich auch für
![Importance of Whey Protein in Wound Healing]( "Importance of Whey Protein in Wound Healing")
Importance of Whey Protein in Wound Healing55,99 €![Extracellular Hsp90 in Diabetic Wound Healing]( "Extracellular Hsp90 in Diabetic Wound Healing")
Extracellular Hsp90 in Diabetic Wound Healing24,99 €![Wound Regeneration and Repair]( "Wound Regeneration and Repair")
Wound Regeneration and Repair104,99 €![The Molecular and Cellular Biology of Wound Repair]( "The Molecular and Cellular Biology of Wound Repair")
The Molecular and Cellular Biology of Wound Repair38,99 €![The Molecular and Cellular Biology of Wound Repair]( "The Molecular and Cellular Biology of Wound Repair")
The Molecular and Cellular Biology of Wound Repair220,99 €![Antioxidants of Healing Herbs: Withania and Rauwolfia]( "Antioxidants of Healing Herbs: Withania and Rauwolfia")
Antioxidants of Healing Herbs: Withania and Rauwolfia32,99 €![Healing]( "Healing")
Healing25,99 €
Skin cell migration is essential for skin wound
healing. Steps for cell migration are often
disrupted in non-healing wounds, causing patient
morbidity. Currently-available
treatments are unsatisfactory. To identify novel
wound-healing targets, we studied the migratory gene
profiles in human keratinocytes (HKs). The main
challenge of this study is to separate genes that
are often simultaneously induced by pleiotropic
signals of a growth factor, including
migration, proliferation and metabolism. Therefore,
we designed the following steps. First, we took
advantage of a unique response of HKs to TGF-beta,
which inhibits proliferation but not migration of
the cells, to suppress selectively the proliferation
signal-responding genes. Second, we independently
stimulated HKs with TGF-alpha or insulin to identify
the commonly regulated genes and eliminate TGF-alpha-
or insulin-specific genes. Under these designs, we
obtained the profiles of early genes by microarray
analyses, followed by QRT-PCR validation and
subsequently functional characterizations by RNAi.
The study suggested the importance of secretory
molecules in keratinocyte migration.
healing. Steps for cell migration are often
disrupted in non-healing wounds, causing patient
morbidity. Currently-available
treatments are unsatisfactory. To identify novel
wound-healing targets, we studied the migratory gene
profiles in human keratinocytes (HKs). The main
challenge of this study is to separate genes that
are often simultaneously induced by pleiotropic
signals of a growth factor, including
migration, proliferation and metabolism. Therefore,
we designed the following steps. First, we took
advantage of a unique response of HKs to TGF-beta,
which inhibits proliferation but not migration of
the cells, to suppress selectively the proliferation
signal-responding genes. Second, we independently
stimulated HKs with TGF-alpha or insulin to identify
the commonly regulated genes and eliminate TGF-alpha-
or insulin-specific genes. Under these designs, we
obtained the profiles of early genes by microarray
analyses, followed by QRT-PCR validation and
subsequently functional characterizations by RNAi.
The study suggested the importance of secretory
molecules in keratinocyte migration.