The crude methanolic extract and different fractions of the whole plant of Gomphrena globosa(L) were subjected to phytochemical screening.The whole plant of G. globosa was extracted with methanol by cold extraction.From the concentrated methanolic crude extract Stigmasterol, beta Sitosterol and Isochavicinic acid were isolated with the help of different standard separating techniques.The isolated compounds were characterized by 1HNMR data and compared with authentic published data.Herewith Isochavicinic acid was isolated and characterized by 1HNMR and 2DNMR data for the first time from this plant.