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In this study the in vitro culture of a range of Daphne species was studied in order to determine whether a genotype-independent micropropagation regime could be identified. Daphnes are ornamental plants and include a range of medicinal compounds of great importance; novel problems such as appearance of Daphne mosaic virus (DapMV) could potentially be eliminated via tissue culture. Six species were successfully established in basal medium. A single micropropagation protocol with some modifications for each species has been developed.