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Methyl tert-Butyl Ether (MTBE) is the most commonly used fuel oxygenate in the world. Its recalcitrant nature as well as its chemical properties have led to widespread groundwater contamination. Questions regarding its toxicity have spurred a search for viable oxygenate alternatives. Since biodegradability is a key indicator of a chemical's environmental impact, this research used three different well-known methods, BOD5, respirometry, and GC analysis, to examine the extent and rates of aerobic biodegradation of MTBE along with tert-butyl alcohol (TBA). The common fuel component toluene was added to some of the samples to determine if the presence of a co-contaminant would effect aerobic microbial degradation of TBA or MTBE.