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From this book you will learn: How to develop an HPLC method for quantitative analysis of 3-methyl-4-nitrophenol, How to validate an HPLC method, How to collect urine samples, How to extract 3-methyl-4-nitrophenol for HPLC analysis, How to quantify 3-methyl-4-nitrophenol using the developed HPLC method In addition, the book include a very brief introduction about Fenitrothion and its metabolism, summaries on the physico-chemical properties and toxicology of 3-methyl-4-nitrophenol and analysis of metabolite in drinks.