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Cancer is the second leading cause of death in the world after cardiovascular diseases. Today, millions of cancer people extend their life due to early identification and treatment. Breast cancer is one of the most common and serious malignancies worldwide. Despite intensive cancer control efforts, it remains the second-leading cause of cancer death among women. MCF-7 cells are useful for in vitro breast cancer studies because the cell line has retained several ideal characteristics particular to the mammary epithelium. High-throughput and high-content analysis of cultured cells or even small organisms are of imminent importance in drug discovery, toxicology and in individualized medicine. In medicine, the identification of tailored patient-specific therapy is important in cancer, chronic inflammation, infection and other diseases . The mechanism of action of anti-proliferative gold complexes had been under question for a long time, However reports that auranofin and other gold(I) complexes potently inhibited the enzyme TrxR (Thioredoxin Reductase) have most probably answered the question on the biological main target of gold complexes.