In the present work the dielectric formalism like Plasmon Pole Approximation (PPA) has been used to calculate the probability for an energetic proton and -particle to produce electronic excitations in two targets of high biological interest, namely, liquid water and DNA . Also, the effects of ionization fraction calculate the Partial Stopping Power Effective Charge (PSPEC) of ( Au, C, Al, Cs) , liquid water and DNA has been studied taking in to consideration electronic excitation in the target. Bragg rule has been used in calculation, but there is a need to pay more attention to investigate the kind of bond on energy loss.