Effect of growth regulators on tomato (Lycopersicon esculentum Mill.)
On shoot induction and plant regeneration in Lycopersicon esculentum Mill
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Four locally grown varieties of tomato; BINA-3,BARI-3,Bahar,Maple and one Indian variety; Pussa Rubby (PR) were selected in this study to observe the effects of growth regulators(in different concentrations&combinations)on shoot initiation and root induction. MS media containing 1.5mg/l BAP and 0.2mg/l IAA showed best result with lowest callus formation and increased number of shoot in all the varieties.In this medium the best response was found for Maple(93.33%)followed by Pussa Ruby(86.67%)and for the other varieties such as BARI-3,BINA-3& Bahar gave almost similar response to that combinati...
Four locally grown varieties of tomato; BINA-3,BARI-3,Bahar,Maple and one Indian variety; Pussa Rubby (PR) were selected in this study to observe the effects of growth regulators(in different concentrations&combinations)on shoot initiation and root induction. MS media containing 1.5mg/l BAP and 0.2mg/l IAA showed best result with lowest callus formation and increased number of shoot in all the varieties.In this medium the best response was found for Maple(93.33%)followed by Pussa Ruby(86.67%)and for the other varieties such as BARI-3,BINA-3& Bahar gave almost similar response to that combination of the medium.The survival response of all varieties was in between 80-90%..BINA-3 showed highest number of fruits per plant while BINA-3 and BARI-3 showed highest average number of seeds per fruit.Finally,it was found that 1.5 mg/l BAP with 0.2mg/l IAA containing MS media is better for shoots induction and 0.2mg/l IAA containing strength MS media is better for root formation in all five varieties.Each experiment was conducted three times(n=3).Regeneration percentages were analyzed by using SPSS(version 16)& significant differences among means were assessed by the Duncan test(P0.05).
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