Arthur R. Schulz

Enzyme Kinetics

From Diastase to Multi-Enzyme Systems

• Broschiertes Buch

Produktbeschreibung

This textbook for advanced courses in enzyme chemistry and enzyme kinetics covers the field of steady state enzyme kinetics from the basic principles inherent in the Henri equation to the expressions which describe the control of multi-enzyme pathways. Steady state kinetic equations are derived with the use of the connection matrix method, and an algorithm which can be implemented easily for computer-based derivation of the equations is developed. Throughout the book emphasis is placed on a proper interpretation of the kinetic behavior of multi-reactant enzymic reactions. The principles of analytical geometry and basic calculus are employed to analyze non-hyperbolic substrate saturation curves in terms of the mechanisms, including subunit interactions, which cause the deviation from hyperbolic curves. Sensitivity theory is employed to obtain expressions for flux and concentration control coefficients for multi-enzyme pathways. The algorithm developed can be incorporated into a computer-based program for derivation. Emphasis is placed on the interpretation of control coefficients in terms of metabolic control. Problems are included at the end of each chapter and their solutions are found at the end of the book.

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Produktdetails

• Verlag: Cambridge University Press
• Seitenzahl: 260
• Erscheinungstermin: 9. Juni 2003
• Englisch
• Abmessung: 229mm x 152mm x 15mm
• Gewicht: 427g
• ISBN-13: 9780521449502
• ISBN-10: 0521449502
• Artikelnr.: 21135380

Inhaltsangabe

Preface
Part I. Basic Steady State Enzyme Kinetics: 1. Derivation of a rate equation
2. A closer look at the basic assumptions
3. Enzyme inhibition
4. Reversible enzyme-catalyzed reactions
Part II. Enzyme Reaction Sequence: 5. Multi-reactant enzymic reactions
6. Analysis of multi-reactant enzyme kinetics
7. Prediction of reaction sequence
8. Enzyme-catalyzed isotopic exchange
9. Kinetic isotope effect on steady state parameters
10. The effect of pH on enzyme kinetics
Part III. Non-Hyperbolic Enzyme Kinetics: 11. The causes of non-hyperbolic enzyme kinetics
12. Analysis of non-hyperbolic enzyme kinetics
13. The effect of subunit interactions on enzyme kinetics
Part IV. Control of Multi-Enzyme Systems: 14. Control of linear multi-enzyme systems
15. Control of branched multi-enzyme systems
16. Biochemical systems theory
Part V. Solutions To Problems: Author index
Subject index.