Chitosan is a natural biopolymer that is derived from chitin, a major component of crustacean outer skeletons . Chitosan is derived from partially deacetylated chitin and consists of copolymers of glucosamine and N-acetyl glucosamine. It has many amino groups attached on the polysaccharide main chain that are readily available for chemical reaction and salt formation with acids . Chitosan has been using in a variety of fields such as wastewater treatment, medicine, agriculture, food, paper industry and cosmetics. In the last 20 years chitosan has drawn considerable attention in biomedical areas, such as wound dressings , cholesterol-lowering agent, hemostatic agent], skin-grafting template, and drug delivery systems.