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Fenugreek (Trigonella Foenum-Graecum)has many uses as herbal medicine, and different fenugreek extraction forms have been tested for therapeutic uses, however data on acute toxicity and safety of fenugreek still not sufficient. Therefore, toxicological evaluation of different forms of fenugreek extracts in experimental animals is a prerequisite for clinical trials, and future therapeutic applications. The objective of this study was to study the acute toxicity of fenugreek seeds aqueous extract in vivo.