With the advent of technology in educational context, the utilization of e-portfolios has become more common in the last decades. Many researchers investigated the role of e-portfolios in assessment area on different skills and levels. E-portfolios offer students and instructors numerous advantages over traditional means of assessment, including a means by which progress can be tracked longitudinally. Analyzing the scores obtained in this study revealed the significant effect of e-portfolio assessment on writing ability and its significant superiority over its counterpart traditional method of assessment. The results also showed that e-portfolio application had statistically significant effect on long-term retention of grammatical points and maintenance of learning. The results of this study are beneficial for both the students and the teachers. Assessment through e-portfolio provides a safe learning environment in which students involve in the process of their learning while theburden of direct teaching and assessment on the shoulder of teachers become milder.