Biochemical Investigation into the HNH Motif of HK97 gp74 - Hyder, Batool Z.
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Bacteriophages are viruses that infect bacteria. This book describes studies of gp74 from the bacteriophage HK97, which functions as an HNH endonuclease. HNH endonucleases are DNA digestion proteins characterized by two highly conserved His residues and an Asn residue. Like other HNH endonucleases, the activity of gp74 is dependent on binding of divalent metal ions to the HNH motif. Current work focused on confirming the identity of conserved HNH motif residues of gp74. We hypothesized the catalytic His residue is H43, the structural Asn residue is N73, and that H82 is involved in…mehr

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Produktbeschreibung
Bacteriophages are viruses that infect bacteria. This book describes studies of gp74 from the bacteriophage HK97, which functions as an HNH endonuclease. HNH endonucleases are DNA digestion proteins characterized by two highly conserved His residues and an Asn residue. Like other HNH endonucleases, the activity of gp74 is dependent on binding of divalent metal ions to the HNH motif. Current work focused on confirming the identity of conserved HNH motif residues of gp74. We hypothesized the catalytic His residue is H43, the structural Asn residue is N73, and that H82 is involved in metal-binding. Additional residues in the betabeta fold, such as D42, may also bind the metal. Our bound metal analysis and the sequence of gp74 also suggest the presence of a Zn2+-finger motif. Mutations of D42 and H82 decrease the activity of gp74, without affecting the structure. These studies advance our understanding of the gp74 activity.
Autorenporträt
HBSc (University of Toronto) in Molecular Biology specialist & double majors in Chemistry and Health Sciences. MSc with departments of Cells & Systems Biology and Chemical & Physical Sciences. Research focus: HK97 gp74 and its HNH motif using several molecular and biochemical techniques. Interests: Cake decoration, baking, painting, and writing.