The present experiment was conducted in the Central farm OUAT during Rabi season 2014-15 with five sesame varieties viz. Uma, Amrit, Smarak, Prachi & Nirmala to study the efficiency of growth regulators such as NAA, GA & IAA in two Conc. (10 ppm & 20 ppm ) sprayed at 30 & 45 DAS. The experiment was laid out in split-plot design with three replications. The morpho - physiological and bio-chemical observations were recorded at different stages of growth of all the varieties due to effect of growthregulators. Significant increase was observed in response to growth regulator on germination of seeds and the highest percentage of seed germination was recorded in GA3 20 ppm. Due to spraying of growth regulators there was increase of plant height, number of branches & leaf area per plant in all the treatments irrespective of varieties and the highest value was exhibited by Nirmala as regards to above characters. The chlorophyll content both chlorophyll-a, chlorophyll-b and total chlorophyll increased due to spraying growth regulators over the control. The LAI, RGR, NAR, CGR & LAD were found to be increased in PGRs treatments over the control.