Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus - Shrivastava, Neeraj;Sharma, Bhaskar;Shrivastav, Arpita
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The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which…mehr

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Produktbeschreibung
The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low.
Autorenporträt
Dr Neeraj Shrivastava (Ph.D) is working as Assistant Professor in the Department of Veterinary Microbiology at N.D.V.S.U.,Jabalpur(M.P.)The present work is his MVSc thesis.Dr Shrivastava is a gold medalist in B.V.Sc & A.H.He has various research articles in his credit.At present he is associated with antimicrobial resistance and MRSA.