A- Mastitis is a major problem in the field that cause huge economical loss.What is the solution?Due to very difficult treatment of mastitis especially with high pathogenicity Microorganisms we use Nanotechnology to solve this problemB- Tumor is still a major problem and till now there is no effective and safe treatment for the problem , so we use Nanotechnology to solve this problem C- Horses suffering from metritis is a major problem in horse deseases and its diagnosis is importantD- Coxiella burnetii affect human and animal. There is no enough researches about this microorganism. We found that we must investigate this microorganism.